Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey

ULUTAŞ, Gürol; TANELİ, FATMA; TÜZÜN, Çiğdem; TIKIZ, CANAN; Ulman, Cevval; DAYAN, İdris

Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey

İdris DAYAN, (Tunceli Devlet Hastanesi, Fiziksel Tıp ve Rehabilitasyon Bölümü, Tunceli, Türkiye)

Canan TIKIZ, (Celal Bayar Üniversitesi, Tıp Fakültesi, Fiziksel Tıp ve Rehabilitasyon Anabilim Dalı, Manisa, Türkiye)

Fatma TANELİ, (Celal Bayar Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Manisa, Türkiye)

Cevval ULMAN, (Celal Bayar Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Manisa, Türkiye)

Gürol ULUTAŞ, (Celal Bayar Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Manisa, Türkiye)

Çiğdem TÜZÜN (Celal Bayar Üniversitesi, Tıp Fakültesi, Fiziksel Tıp ve Rehabilitasyon Anabilim Dalı, Manisa, Türkiye)

Turkish Journal of Rheumatology(.)Archives of Rheumatology

2 0

Yıl: 2010 Cilt: 25 Sayı: 1 Sayfa Aralığı: 12 - 18 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey

Öz:

Amaç: HLA DRB1 ortak epitop alelleri romatoid artritin (RA) en belirgin genetik risk faktörü olup, sınıf 2 HLA molekülünün peptid sunan bölümünde bir amino asit dizilimi tarafından kodlanmaktadır Son zamanlarda bu ortak epitop alellerinin RA'lı hastalarda sitrüllenmiş proteinlere karşı antikorların gelişimi için bir risk faktörü olabileceği öne sürülmektedir. Bu çalışmada Türk toplumundaki RA'lı hastalarda siklik sitrüline peptid antikorları (anti-CCP) pozitifliğinin HLA-DRB1 allellerinin taşıdığı ortak epitopla olan ilişkisi araştırılmıştır.Gereç ve Yöntem: Çalışmaya kliniğimizde yeni tanı konulmuş ya da tedavi altında olan 60 RA'lı hasta dahil edildi. Kontrol grubunu ise 60 sağlıklı gönüllü oluşturdu. RA'lı hastalarda anti-CCP düzeyleri enzim-bağımlı immunosorbent ölçüm metoduyla, HLA-DRB1 tiplemeleri ve OE ölçümü polimeraz zincir reaksiyonu yöntemi ile araştırıldı. Sağlıklı gönüllülerde ise sadece anti-CCP düzeyleri ölçüldü.Bulgular: Romatoid artritli hastalarda OE pozitifliği %50 olarak saptandı ve bu hastaların %30'u çift kopya OE taşıyordu. Anti-CCP pozitifliği RA'lı hastalarda %73.3 iken, kontrol grubunda bu oran %0 olarak saptandı. OE varlığının anti-CCP pozitifliğini arttırdığı (OR=4.3, %95 [CI], P=0.04 ) ve anti-CCP pozitifliği ile RF pozitifliği arasında anlamlı bir ilişkisi olduğu gözlendi (OR=5.3, %95 [CI] P<0.05). Sonuç: Çalışmamızda Türk RA'lı hastalarda OE taşıyan HLA-DRB1 genlerinin anti-CCP üretimiyle anlamlı ilişkisi olduğu ve antiCCP'nin RA için tanısal duyarlılık ve özgüllüğünün sırasıyla %73.3 ve %100 olduğu saptanmıştır.

Anahtar Kelime:

Konular: Romatoloji

Türk Romatoid Artritli Hastalarda Siklik Sitrüline Peptit Antikor Pozitifliği ile HLA-DRB1 Ortak Epitop Alelleri Arasındaki ilişki

Öz:

Objective: The most characteristic genetic risk factors for rheumatoid arthritis (RA), the HLA-DRB1 shared epitope (SE) alleles, encode for a common amino acid sequence in the peptide-presenting part of the HLA class II molecule. These SE alleles have been described recently to be a risk factor for the development of antibodies against citrullinated proteins in RA. The current study was performed to investigate the association between the cyclic citrullinated peptide antibodies (anti-CCP) and HLA-DR1 HLA-DRB1 shared epitope alleles in patients with RA in Turkey.Materials and Methods: Sixty patients with RA who were newly diagnosed or under conventional treatment in our clinic and 60 healthy volunteers as controls were enrolled in the study. In patients with RA anti-CCP levels were investigated with enzymelinked immunosorbent assay and HLA-DRB1 subtyping and SE was assessed by polymerase chain reaction. Only anti-CCP was measured in healthy volunteers.Results: SE was positive in 50% of the patients with RA. Amongst the SE carriers, 30% of them were carrying double copy of SE. While anti-CCP was positive in 73,3% of patients with RA, this ratio was 0% in healthy volunteers. We determined that the existence of SE increases the positivity of anti-CCP (OR=4,3, 95% [CI], P=0.04 ), and a significant relationship was found between the anti-CCP positivity and the RF positivity. (OR=5,3, 95% [CI] P<0.05). Conclusion: The results of the present study revealed that Turkish patients with RA carrying SE with HLA-DRB1 genes is significantly related with the production of anti-CCP. The diagnostic sensitivity and specificity of anti-CCP for RA is determined as 73,3% and 100% respectively.

Anahtar Kelime:

Konular: Romatoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	DAYAN İ, TIKIZ C, TANELİ F, Ulman C, ULUTAŞ G, TÜZÜN Ç (2010). Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. , 12 - 18.
Chicago	DAYAN İdris,TIKIZ CANAN,TANELİ FATMA,Ulman Cevval,ULUTAŞ Gürol,TÜZÜN Çiğdem Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. (2010): 12 - 18.
MLA	DAYAN İdris,TIKIZ CANAN,TANELİ FATMA,Ulman Cevval,ULUTAŞ Gürol,TÜZÜN Çiğdem Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. , 2010, ss.12 - 18.
AMA	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. . 2010; 12 - 18.
Vancouver	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. . 2010; 12 - 18.
IEEE	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç "Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey." , ss.12 - 18, 2010.
ISNAD	DAYAN, İdris vd. "Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey". (2010), 12-18.

APA	DAYAN İ, TIKIZ C, TANELİ F, Ulman C, ULUTAŞ G, TÜZÜN Ç (2010). Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. Turkish Journal of Rheumatology(.)Archives of Rheumatology, 25(1), 12 - 18.
Chicago	DAYAN İdris,TIKIZ CANAN,TANELİ FATMA,Ulman Cevval,ULUTAŞ Gürol,TÜZÜN Çiğdem Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. Turkish Journal of Rheumatology(.)Archives of Rheumatology 25, no.1 (2010): 12 - 18.
MLA	DAYAN İdris,TIKIZ CANAN,TANELİ FATMA,Ulman Cevval,ULUTAŞ Gürol,TÜZÜN Çiğdem Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. Turkish Journal of Rheumatology(.)Archives of Rheumatology, vol.25, no.1, 2010, ss.12 - 18.
AMA	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. Turkish Journal of Rheumatology(.)Archives of Rheumatology. 2010; 25(1): 12 - 18.
Vancouver	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey. Turkish Journal of Rheumatology(.)Archives of Rheumatology. 2010; 25(1): 12 - 18.
IEEE	DAYAN İ,TIKIZ C,TANELİ F,Ulman C,ULUTAŞ G,TÜZÜN Ç "Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey." Turkish Journal of Rheumatology(.)Archives of Rheumatology, 25, ss.12 - 18, 2010.
ISNAD	DAYAN, İdris vd. "Relationship Between Cyclic Citrullinated Peptide Antibodies Positivity and HLA-DRB1 Shared Epitope Alleles in Patients with Rheumatoid Arthritis in Turkey". Turkish Journal of Rheumatology(.)Archives of Rheumatology 25/1 (2010), 12-18.