Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması

KHALIQ, Kashfa; AKHTAR, Masood; AWAIS, Mian Muhammad; ANWAR, Muhammad Irfan

Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması

Kashfa KHALIQ, (Department of Microbiology, Govt. College University, Faisalabad, PAKISTAN)

Masood AKHTAR, (Department of Parasitology, University of Agriculture, Faisalabad, PAKISTAN)

Mian Muhammad AWAIS, (Bahauddin Zakariya University)

Muhammad Irfan ANWAR (Department of Pathobiology, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, PAKISTAN)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

40 0

Yıl: 2017 Cilt: 23 Sayı: 6 Sayfa Aralığı: 895 - 901 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması

Öz:

Bu çalışma ile tavuklarda koksidiyoza karşı Aloe (A.) vera polisakkaritlerinin immunoterapötik etkisi rapor edilmektedir. Bu amaçla, A. vera'dan polisakkaritler elde edildi ve HPLC ile analizleri yapıldı. Maltoz, glikoz ve mannoz içeren üç farklı heksoz şekeri A. vera'nın hidrolize edilmiş solüsyonunda belirlendi. Ekstrakte edilen polisakkaritlerin (dereceli dozlarda) tavuklarda koksidiyoza karşı immunoterapötik etkisi araştırıldı. Sonuçlar; yüzde koruma ve günlük ağırlık kazanımının A. vera polisakkaritleri verilen tavuklarda kontrol grubuna oranla istatistiksel olarak daha yüksek olduğunu gösterdi (P<0.05). Oosit sayısı ve lezyon skoru ise polisakkarit verilen tavuklarda kontrol grubuna oranla istatistiksel olarak daha düşüktü (P<0.05). Anti koksidial belirtiler de polisakkarit verilen tavuklarda (159.75-239.63) kontrol grubuna oranla (36.57) daha yüksekti. Dalak dışındaki tüm lenfoid organların organ-vücut ağırlığı oranları deney ve kontrol grubundaki hayvanlarda benzerlik göstermekteydi (P>0.05). Çalışmanın bulgularına dayanılarak, A. vera polisakkaritlerinin tavuklarda koksidiyoza karşı immunoterapötik eksinin olduğu ve bu nedenle tavuklarda koksidiyozu kontrol altında tutabilmek amacıyla ticari kullanımının araştırılması gerektiği sonucuna varılmıştır.

Anahtar Kelime:

Konular: Veterinerlik

Evaluation of Immunotherapeutic Effects of Aloe vera Polysaccharides Against Coccidiosis in Chicken

Öz:

This study reports the immunotherapeutic effects of Aloe (A.) vera polysaccharides against coccidiosis in broiler chicken. For the purpose, polysaccharides were recovered from A. vera and analyzed by using HPLC. Three different hexose sugars including maltose, glucose and mannose were detected in hydrolyzed solution of A. vera. The extracted polysaccharides (graded doses) were evaluated for immunotherapeutic activities against coccidiosis in chicken. Results revealed that percent protection and daily weight gains were significantly higher (P<0.05) in chicken administered with A. vera polysaccharides as compared to control group. On the other hand, oocyst counts and lesion scores were lower (P<0.05) in polysaccharides administered chickens as compared to control. Moreover, anti-coccidial indices were also higher in chickens administered with polysaccharides (159.75-239.63) as compared to control (36.57). Except spleen, the organ-body weight ratios of all lymphoid organs of experimental and control groups were statistically similar (P>0.05). Based upon findings of this study, it was concluded that A. vera derived polysaccharides had immunotherapeutic activity against coccidiosis in chickens and might be further explored for its commercial feasibility for effective use in poultry industry to control avian coccidiosis.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Kostadinovic LJ, Puvaca N, Popovic S, Levic J: Botanical supplements as anti-coccidial alternatives in poultry nutrition. World Poult Sci J, 71, 27-36, 2015. DOI: 10.1017/S0043933915000033
2. Khan MQ, Irshad H, Anjum R, Jahangir M, Nasir U: Eimeriosis in poultry of Rawalpindi/Islamabad area. Pak Vet J, 26, 85-87, 2006.
3. Kaboudi K, Umar S, Munir MT: Prevalence of coccidiosis in free range chicken in Sidi Thabet, Tunisia. Scientifica, 2016 (2016), Article ID: 7075195. DOI: 10.1155/2016/7075195
4. Dalloul RA, Lillehoj HS: Poultry coccidiosis: recent advancements in control measures and vaccine development. Expert Rev Vacc, 5, 143-163, 2006. DOI: 10.1586/14760584.5.1.143
5. Awais MM, Akhtar M, Muhammad F, Haq AU, Anwar MI: Immunotherapeutic effects of some sugar cane (Saccharum officinarum L.) extracts against coccidiosis in industrial broiler chickens. Exp Parasitol, 128, 104-110, 2011. DOI: 10.1016/j.exppara.2011.02.024
6. Williams RB: Tracing the emergence of drug-resistance in coccidia (Eimeria sp.) of commercial broiler flocks medicated with decoquinate for the first time in the United Kingdom. Vet Parasitol, 135, 1-14, 2006. DOI: 10.1016/j.vetpar.2005.10.012
7. Allen PC, Fetterer RH: Recent advances in biology and immunobiology of Eimeria species and in diagnosis and control of infection with these coccidian parasites of poultry. Clin Microbiol Rev, 15, 58-65, 2002. DOI: 10.1128/CMR.15.1.58-65.2002
8. Kitandu A, Juranova R: Progress in control measures for chicken coccidiosis. Acta Veterinaria Brno, 75, 265-276, 2006. DOI: 10.2754/ avb200675020265
9. Dalloul RA, Lillehoj HS, Lee JS, Lee SH, Chung KS: Immunopotentiating effect of a Fomitella fraxinea derived lectin on chicken immunity and resistance to coccidiosis. Poult Sci, 85, 446-451, 2006. DOI: 10.1093/ ps/85.3.446
10. Ullah MI, Akhtar M, Iqbal Z, Shahid M, Awais MM: Immunomodulating and antiprotozoal effects of different extracts of the oyster culinary medicinal mushroom Pleurotus ostreatus (Higher Basidiomycetes) against coccidiosis in broiler. Int J Med Mushrooms, 17, 309-317, 2015. DOI: 10.1615/IntJMedMushrooms.v17.i3.100
11. Krishnan P: The scientific study of herbal wound healing therapies: Current state of play. Curr Anaesth Crit Care, 17, 21-27, 2006.DOI: 10.1016/j. cacc.2006.02.009
12. Akhtar M, Hai A, Awais MM, Iqbal Z, Muhammad F, Haq A, Anwar MI: Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens. Vet Parasitol, 186, 170-177, 2012. DOI: 10.1016/j.vetpar.2011.11.059
13. Tan ZJ, Li FF, Xu XL, Xing JM: Simultaneous extraction and purification of aloe polysaccharides and proteins using ionic liquid based aqueous two-phase system coupled with dialysis membrane. Desalination 286, 389-393, 2012. DOI: 10.1016/j.desal.2011.11.053
14. Aysan E, Bektas H, Ersoz F: A new approach to postoperative peritoneal adhesions: prevention of peritoneal trauma by Aloe vera gel. Eur J Obstet Gynecol Reprod Biol, 149, 195-198, 2010. DOI: 10.1016/j. ejogrb.2009.11.019
15. Ni Y, Turner D, Yates KM, Tizard I: Isolation and characterization of structural components of Aloe vera L. leaf pulp. Int Immunopharmacol, 4, 1745-1755, 2004. DOI: 10.1016/j.intimp.2004.07.006
16. Chantarawarati P, Sangvanich P, Banlunara W, Soontornvipart K, Thunyakitpisal P: Acemannan sponges stimulate alveolar bone, cementum and periodontal ligament regeneration in a canine class II furcation defect model. J Periodontal Res, 49, 164-178, 2014. DOI: 10.1111/ jre.12090
17. Duansak D, Somboonwong J, Patumraj S: Effect of Aloe vera on leukocyte adhesion and TNF-a and IL-6 levels in burn wounded rats. Clin Hemorheol Microcircul, 29, 239-246, 2009.
18. Im SA, Oh ST, Song S, Kim MR, Kim DS, Woo SS, Jo YI, Lee CK: Identification of optimal molecular size of modified Aloe polysaccharides with maximum immunomodulatory activity. Int Immunopharmacol, 5, 271-279, 2005. DOI: 10.1016/j.intimp.2004.09.031
19. Khaliq K, Akhtar M, Iqbal Z, Hussain I: Aloe vera polysaccharides as biological response modifiers in chickens. Int J Agric Biol, 18, 274- 281, 2016. DOI: 10.17957/IJAB/15.0077
20. Chang XL, Chen BY, Feng YM: Water soluble polysaccharides isolated from skin juice, gel juice and flower of Aloe vera Miller. J Taiwan Inst Chem Eng, 2, 197-203, 2010. DOI: 10.1016/j.jtice.2010.07.007
21. Staub AM: Removal of protein-Sevag method. Methods Carbohydr. Chem. 5, 5-6, 1965.
22. Osborn HMI, Lochey F, Mosley L, Read D: 1999. Analysis of polysaccharides and monosaccharides in the root mucilage of maize (Zea mays L.) by gas chromatography. J Chromatogr, 831, 267-276, 1999. DOI: 10.1016/S0021-9673(98)00935-2
23. Anwar MI, Akhtar M, Hussain I, Haq AU, Muhammad F, Hafeez MA, Mahmood MS, Bashir S: Field evaluation of Eimeria tenella (local isolates) gametocytes vaccine and its comparative efficacy with imported
24. Johnson J, Reid WM: Anticoccidial drugs: Lesion scoring techniques in battery and floor pen experiments with chickens. Exp Parasitol, 28, 30- 36, 1970. DOI: 10.1016/0014-4894(70)90063-9
25. Singh J, Gill BS: Effect of gamma-irradiation on oocysts of Eimeria necatrix. Parasitology, 71, 117-124, 1975. DOI: 10.1017/S0031182000053208
26. Shah MAA, Song X, Xu L, Yan R, Song H, Ruirui Z, Chengyu L, Li X: The DNA-induced protective immunity with chickens interferon gamma against poultry coccidiosis. Parasitol Res, 107, 747-750, 2009. DOI: 10.1007/s00436-010-1940-9
27. Shah MMA, Xu L, Yan R, Song X, Li X: Cross immunity of DNA vaccine pVAX1-cSZ2-IL-2 to Eimeria tenella, E. necatrix and E. maxima. Exp Parasitol, 124, 330-333, 2010. DOI: 10.1016/j.exppara.2009.11.010
28. Peek HW, Landman WJM: Resistance to anticoccidial drugs of Dutch avian Eimeria spp. field isolates originating from 1996, 1999 and 2001. Avian Pathol, 32, 391-401, 2003. DOI: 10.1080/0307945031000121149
29. Kaleem QM, Akhtar M, Awais MM, Saleem M, Zafar M, Iqbal Z, Muhammad F, Anwar MI: Studies on Emblica officinalis derived tannins for their immunostimulatory and protective activities against coccidiosis in industrial broiler chickens. Scientific World J, 2014, 1-10, 2014. DOI: 10.1155/2014/378473
30. Altug N, Yuksek N, Agaoglu ZT: Immunostimulating effects of Aloe vera and glucan on cellular and humoral immune responses following vaccination with polyvalent vaccines in dogs. Kafkas Univ Vet Fak Derg, 16, 405-412, 2010. DOI: 10.9775/kvfd.2009.842
31. Rajasekaran S, Sivagnanam K, Subramanian S: Antioxidant effects of Aloe vera gel extract in streptozocin-induced diabetes in rats. Pharm Rep, 57, 90-96, 2005.
32. Choi S, Chung MH: A review on the relationship between Aloe vera components and their biological effects. Sem Integ Med, 1, 53-62, 2003. DOI: 10.1016/S1543-1150(03)00005-X
33. Moghaddasi SM, Verma SK: Aloe vera their chemicals composition and applications: A review. Int J Biol Med Res, 2, 466-471, 2011.
34. Chang XL, Wang C, Feng Y, Liu Z: Effects of heat treatments on the stabilities of polysaccharides substances and barbaloin in gel juice from Aloe vera Miller. J Food Eng, 75, 245-251, 2006. DOI: 10.1016/j. jfoodeng.2005.04.026
35. Chow JTN, Williamson DA, Yates KM, Goux WJ: Chemical characterization of the immunomodulating polysaccharide of Aloe vera. Carbohydr Res, 340, 1131-1142, 2005. DOI: 10.1016/j.carres.2005.02.016
36. Reynolds T, Dweck AC: Aloe vera leaf gel: A review update. J Ethnopharmacol, 68, 3-37, 1999. DOI: 10.1016/S0378-8741(99)00085-9
37. Shokraneh M, Ghalamkari G, Toghyani M, Landy N: Influence of drinking water containing Aloe vera (Aloe barbadensis Miller) gel on growth performance, intestinal microflora, and humoral immune responses of broilers. Vet World, 9, 1197-1203, 2016. DOI: 10.14202/ vetworld.2016.1197-1203
38. Yim D, Kang SS, Kim DW, Kim SH, Lillehog HS, Min W: Protective effects of Aloe vera based diets in Eimeria maxima infected broiler chickens. Exp Parasitol, 127, 322-325, 2011. DOI: 10.1016/j.exppara.2010.08.010
39. Sarı B, Cakmak A: Preventive effects of anticoccidial drug supplementation in chicken feed against coccidiosis in broiler farming. Kocatepe Vet Derg, 1, 1-10, 2008.
40. Ozkan O, Sarı B, Bayezit M, Dogan A, Akpulat HA, Erdag D: Anticoccidiosis effect of Thymus serpyllum in rabbits: Oocyst shedding and body weight changes. Kafkas Univ Vet Fak Derg, 16, 323-327, 2010. DOI: 10.9775/kvfd.2009.790
41. Bayezıt M, Sarı B, Atakisi E, Dogan A, Ozkan O, Tasci GT: Anti coccidiosis effect of Thymus Serpyllum in rabbits: Serum NO levels and serum ADA activity. Kocatepe Vet Derg, 3, 7-11, 2010.
42. Arabkhazaeli F, Modrisanei M, Nabian S, Mansoori B, Madani A: Evaluating the resistance of Eimeria Spp. field isolates to anticoccidial drugs using three different Indices. Iran J Parasitol, 8, 234-241, 2013.
43. Waihenya RK, Mtambo MM, Nkwengulila G: Evaluation of the efficacy of the crude extract of Aloe secundiflora in chickens experimentally infected with Newcastle disease virus. J Ethnopharmacol 79, 299-304, 2002. DOI: 10.1016/S0378-8741(01)00370-1
44. Montagne L, Pluske JR, Hampson DJ: A review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals. Anim Feed Sci Technol, 108, 95-117, 2003. DOI: 10.1016/S0377-8401(03)00163-9
45. Darabighane B, Zarei A, Shahneh AZ, Mahdavi A: Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers. Italian J Anim Sci, 10, 189-194, 2011. DOI: 10.4081/ijas.2011.e36
46. Shelton MS: Aloe Vera, its chemical and therapeutic properties. Int J Dermatol, 30, 679-683, 1991. DOI: 10.1111/j.1365-4362.1991. tb02607.x
47. Grindlay D, Reynolds T: The Aloe vera phenomenon: A review of the properties and modern uses of the leaf parenchyma gel. J Ethnopharmacol, 16, 117-156, 1986. DOI: 10.1016/0378-8741(86)90085-1
48. Vahedi G, Taghavi M, Maleki AK, Habibian R: The effect of Aloe vera extract on humoral and cellular immune response in rabbit. Afr J Biotechnol, 10, 5225-5228, 2011.
49. Madan J, Sharma AK, Inamdar N, Rao HS, Singh R: Immunomodulatory properties of Aloe vera gel in mice. Int J Green Pharm, 2, 152- 154, 2008. DOI: 10.4103/0973-8258.42732
50. Cheesbrough M: Medical Laboratory Manual for Tropical Countries. Vol. II. 372-391, Printed and Bond in Great Britain by the University Press, Cambridge. 1984.
51. Pablos LMD, Santos MFBD, Montero E, Garcia-Granados A, Parra A, Osuna A: Anticoccidial activity of maslinic acid against infection with Eimeria tenella in chickens. Parasitol Res,107, 601-604, 2010. DOI: 10.1007/ s00436-010-1901-3
52. Awais MM, Akhtar M, Iqbal Z, Muhammad F: Saccharum officinarum derived mid molecular mass glycoproteins as native BRMs in chickens. Pak J Life Soc Sci, 11, 200-207, 2013.
53. Bettsille MD: Influence of E. acervulina coccidiosis on body and organ development and on serum electrolytes, glucose and total protein in broilers. German Federal Republic, 1405-1406, 1986.

APA	KHALIQ K, AKHTAR M, AWAIS M, ANWAR M (2017). Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. , 895 - 901.
Chicago	KHALIQ Kashfa,AKHTAR Masood,AWAIS Mian Muhammad,ANWAR Muhammad Irfan Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. (2017): 895 - 901.
MLA	KHALIQ Kashfa,AKHTAR Masood,AWAIS Mian Muhammad,ANWAR Muhammad Irfan Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. , 2017, ss.895 - 901.
AMA	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. . 2017; 895 - 901.
Vancouver	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. . 2017; 895 - 901.
IEEE	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M "Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması." , ss.895 - 901, 2017.
ISNAD	KHALIQ, Kashfa vd. "Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması". (2017), 895-901.

APA	KHALIQ K, AKHTAR M, AWAIS M, ANWAR M (2017). Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23(6), 895 - 901.
Chicago	KHALIQ Kashfa,AKHTAR Masood,AWAIS Mian Muhammad,ANWAR Muhammad Irfan Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23, no.6 (2017): 895 - 901.
MLA	KHALIQ Kashfa,AKHTAR Masood,AWAIS Mian Muhammad,ANWAR Muhammad Irfan Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.23, no.6, 2017, ss.895 - 901.
AMA	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(6): 895 - 901.
Vancouver	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2017; 23(6): 895 - 901.
IEEE	KHALIQ K,AKHTAR M,AWAIS M,ANWAR M "Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 23, ss.895 - 901, 2017.
ISNAD	KHALIQ, Kashfa vd. "Tavuklarda Koksidiyoza Karşı Aloe vera Polisakkaritlerinin İmmunoterapötik Etkinliğinin Saptanması". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 23/6 (2017), 895-901.