The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars

GÜRPINAR, Aylin; COMU, Faruk M.; BENDER, R. Ada; Atasoy, Pınar; Yucel, Aykan; NOYAN, Volkan

The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars

R. Ada BENDER, (Kırıkkale University)

Aykan YÜCEL, (Zekai Tahir Burak Women's Health Care Training and Research Hospital, Ankara)

Volkan NOYAN, (Kırıkkale University)

Aylin GÜRPINAR, (Hacettepe University Department ofBiology, Ankara)

Pınar ATASOY, (Kırıkkale University)

Faruk M. COMU (Kırıkkale University)

GORM:Gynecology Obstetrics & Reproductive Medicine

1 0

Yıl: 2017 Cilt: 23 Sayı: 3 Sayfa Aralığı: 120 - 128 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars

Öz:

OBJECTIVE: The mesenchymal stem cell application to uterine healing scars may provide better tissuestrength.STUDY DESIGN: Hysterectomy was performed on rats, and the wound recovery as a result of primarysuturing was evaluated as tissue stretching and the positive histopathological effects. The mesenchymalstem cells originating from the adipose tissue were used during the healing period of the wound and woulddifferentiate to mesenchyme-originated cells present in intact tissue for an optimum level of healing.RESULTS: The weights of non-incised uterine horns in the control group were found to be significantlyhigher than the weights of the incised uterine horns (z=2.52, p=0.012). In the experiment group, theweights of the incised uterine horns were found to be significantly higher than the non-incised uterinehorns (z=2.52, p=0.012). In the control group, the non-incised uterine horns withstood the stretching testto a higher extent than the incised horns, and a significant difference was found between the stretchingvalues (z=2.5, p=0.012). In the experiment group, the incised uterine horns withstood the stretching teststo a higher extent than the non-incised uterine horns; however, there was no significant difference between the stretching tests (z=1.540, p=0.123).CONCLUSION: Adipose tissue-originated mesenchyme stem cells were observed to increase the tissue stretching during wound healing.

Anahtar Kelime:

Konular: Kadın Hastalıkları ve Doğum

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981 Jul 9;292(5819):154-6.
2. Khosrotehrani K Mesenchymal stem cell therapy in skin: why and what for? Exp Dermatol 2013 May;22(5):307- 10.
3. Reyes M, Verfaillie CM. Turning marrow into brain: Generation of Glial and neuronal cells from adult bone marrow mesenchymal stem cells. In Blood 1999 Nov 15; 94(10):377A-377A
4. Horwitz EM, Le Blanc K, Dominici M, Mueller I, SlaperCortenbach I, Marini FC, et al. Clarification of the nomenclature for MSC: The International Society for Cellular Therapy position statement. Cytotherapy 2005 Jan 1; 7(5):393-5.
5. Gimble JM, Bunnell BA, Frazier T, Rowan B, Shah F, Thomas-Porch C, Wu X. Adipose-derived stromal/stem cells: a primer. Organogenesis 2013;9(1):3-10.
6. Ivanova-Todorova E, Bochev I, Dimitrov R, Belemezova K, Mourdjeva M, Kyurkchiev S, Kinov P, Altankova I, Kyurkchiev D. Conditioned medium from adipose tissuederived mesenchymal stem cells induces CD4+FOXP3+ cells and increas IL-10 secretion. J Biomed Biotechnol 2012;2012:295167
7. Arrigoni E, de Girolamo L, Di Giancamillo A, Stanco D, Dellavia C, Carnelli D, et al. Adipose-derived stem cells and rabbit bone regeneration: histomorphometric, immunohistochemical and mechanical characterization. J Orthop Sci 2013 Mar;18(2):331-9.
8. Niyaz M, Gurpinar OA, Gunaydin S, Onur MA. Isolation, culturing and characterization of rat adipose tissue-derived mesenchymal stem cells: a simple technique. T J Biol 2012;36(6):658-64.
9. Lindroos B, Suuronen R, Miettinen S. The potential of adipose stem cells in regenerative medicine. Stem Cell Rev 2011 Jun;7(2):269-91.
10. Darby IA, Hewitson TD. Fibroblast differentiation in wound healing and fibrosis. Int Rev Cytol 2007;257:143- 79.
11. Buhimschi CS, Zhao G, Sora N, Madri JA, Buhimschi IA. Myometrial wound healing post-Cesarean delivery in the MRL/MpJ mouse model of uterine scarring. Am J Pathol 2010 Jul;177(1):197-207.
12. Nakonieczny Z. Conduction of labor following cesarean section. Ginekol Pol 1962 Mar-Apr;33:251-8.
13. Werner S, Grose R. Regulation of wound healing by growth factors and cytokines. Physiol Rev 2003 Jul; 83(3):835-70.
14. Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med 1999 Sep 2;341(10):738-46.
15. Steenfos HH. Growth factors and wound healing. Scand J Plast Reconstr Surg Hand Surg 1994 Jun;28(2):95-105.
16. Bowers D, McKenzie D, Dutta D, Wheeless CR, Cohen WR. Growth hormone treatment after cesarean delivery in rats increases the strength of the uterine scar. Am J Obstet Gynecol 2001 Sep;185(3):614-7.
17. Li X, Sun H, Lin N, Hou X, Wang J, Zhou B, et al. Regeneration of uterine horns in rats by collagen scaffolds loaded with collagen-binding human basic fibroblast growth factor. Biomaterials 2011 Nov;32(32):8172-81.
18. Lin N, Li X, Song T, Wang J, Meng K, Yang J, Hou X, Dai J, Hu Y. The effect of collagen-binding vascular endothelial growth factor on the remodeling of scarred rat uterus following full-thickness injury. Biomaterials 2012 Feb; 33(6):1801-7
19. O'Brien JM, Whetham D, Fecteau C, Jansen J, Hiles M. Augmenting myometrial healing after cesarean delivery: use of an adjuvant biologic graft placement in an ovine model. Am J Perinatol 2011 Aug;28(7):543-50.
20. Nambu M, Ishihara M, Nakamura S, Mizuno H, Yanagibayashi S, Kanatani Y, et al. Enhanced healing of mitomycin C-treated wounds in rats using inbred adipose tissue-derived stromal cells within an atelocollagen matrix. Wound Repair Regen 2007 Jul-Aug;15(4):505-10.
21. Nambu M, Kishimoto S, Nakamura S, Mizuno H, Yanagibayashi S, Yamamoto N, et al. Accelerated wound healing in healing-impaired db/db mice by autologous adipose tissue-derived stromal cells combined with atelocollagen matrix. Ann Plast Surg 2009 Mar;62(3):317-21.
22. Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res 2007 May 11;100(9):1249-60.
23. Yoo JH, Shin JH, An MS, Ha TK, Kim KH, Bae KB, et al. Adipose-tissue-derived Stem Cells Enhance the Healing of Ischemic Colonic Anastomoses: An Experimental Study in Rats. J Korean Soc Coloproctol 2012 Jun; 28(3):132-9.
24. Jun CR, Lee JY, Cho HH. Muscle-derived stem cells and smooth muscle healing in a rat model of uterine injury. Int J Gynaecol Obstet 2012 Mar;116(3):265-6.
25. Maiborodin IV, Yakimova NV, Matveyeva VA, Pekarev OG, Maiborodina EI, Pekareva EO. Angiogenesis in rat uterine cicatrix after injection of autologous bone marrow mesenchymal stem cells. Bull Exp Biol Med 2011 Apr; 150(6):756-61.
26. Zuk PA, Zhu M, Ashjian P, De Ugarte DA, Huang JI, Mizuno H, et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell 2002 Dec;13(12):4279- 95.
27. Rangappa S, Fen C, Lee EH, Bongso A, Sim EK. Transformation of adult mesenchymal stem cells isolated from the fatty tissue into cardiomyocytes. Ann Thorac Surg 2003 Mar;75(3):775-9.
28. Cao Y, Sun Z, Liao L, Meng Y, Han Q, Zhao RC. Human adipose tissue-derived stem cells differentiate into endothelial cells in vitro and improve postnatal neovascularization in vivo. Biochem Biophys Res Commun 2005 Jul 1;332(2):370-9.
29. Katz AJ, Tholpady A, Tholpady SS, Shang H, Ogle RC. Cell surface and transcriptional characterization of human adipose-derived adherent stromal (hADAS) cells. Stem Cells 2005 Mar;23(3):412-23.
30. Francis MP, Sachs PC, Elmore LW, Holt SE. Isolating adipose-derived mesenchymal stem cells from lipoaspirate blood and saline fraction. Organogenesis 2010 Jan-Mar; 6(1):11-4.
31. Baglioni S, Francalanci M, Squecco R, Lombardi A, Cantini G, Angeli R, et al. Characterization of human adult stem-cell populations isolated from visceral and subcutaneous adipose tissue. FASEB J 2009 Oct;23(10): 3494-3505.
32. Kode JA, Mukherjee S, Joglekar MV, Hardikar AA. Mesenchymal stem cells: immunobiology and role in immunomodulation and tissue regeneration. Cytotherapy 2009;11(4):377-91.
33. Ko MS, Jung JY, Shin IS, Choi EW, Kim JH, Kang SK, Ra JC. Effects of expanded human adipose tissue-derived mesenchymal stem cells on the viability of cryopreserved fat grafts in the nude mouse. Int J Med Sci 2011 Mar 14;8(3):231-8.
34. Strem BM, Hicok KC, Zhu M, Wulur I, Alfonso Z, Schreiber RE, et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med 2005 Sep; 54(3):132-41.
35. Ode A, Kopf J, Kurtz A, Schmidt-Bleek K, Schrade P, Kolar P, et al. CD73 and CD29 concurrently mediate the mechanically induced decrease of migratory capacity of mesenchymal stromal cells. Eur Cell Mater 2011 Jul 6;22: 26-42.
36. Wiesmann A, Bühring HJ, Mentrup C, Wiesmann HP. Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation. Head Face Med 2006 Mar 31;2:8.
37. González MA, Gonzalez-Rey E, Rico L, Büscher D, Delgado M. Adipose-derived mesenchymal stem cells alleviate experimental colitis by inhibiting inflammatory and autoimmune responses. Gastroenterology 2009 Mar;136(3):978-89.
38. Spath C, Schlegel F, Leontyev S, Mohr FW, Dhein S. Inverse Relationship between Tumor Proliferation Markers and Connexin Expression in a Malignant Cardiac Tumor Originating from Mesenchymal Stem Cell Engineered Tissue in a Rat in vivo Model. Front Pharmacol 2013 Apr 17;4:42.
39. Ode A, Kopf J, Kurtz A, Schmidt-Bleek K, Schrade P, Kolar P, et al. CD73 and CD29 concurrently mediate the mechanically induced decrease of migratory capacity of mesenchymal stromal cells. Eur Cell Mater 2011 Jul 6;22: 26-42.
40. Wiesmann A, Bühring HJ, Mentrup C, Wiesmann HP. Decreased CD90 expression in human mesenchymal stem cells by applying mechanical stimulation. Head Face Med 2006 Mar 31;2:8.
41. Yang XF, He X, He J, Zhang LH, Su XJ, Dong ZY, Xu YJ, Li Y, Li YL. High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells. J Biomed Sci 2011 Aug 19;18:59

APA	BENDER R, Yucel A, NOYAN V, GÜRPINAR A, Atasoy P, COMU F (2017). The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. , 120 - 128.
Chicago	BENDER R. Ada,Yucel Aykan,NOYAN Volkan,GÜRPINAR Aylin,Atasoy Pınar,COMU Faruk M. The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. (2017): 120 - 128.
MLA	BENDER R. Ada,Yucel Aykan,NOYAN Volkan,GÜRPINAR Aylin,Atasoy Pınar,COMU Faruk M. The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. , 2017, ss.120 - 128.
AMA	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. . 2017; 120 - 128.
Vancouver	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. . 2017; 120 - 128.
IEEE	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F "The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars." , ss.120 - 128, 2017.
ISNAD	BENDER, R. Ada vd. "The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars". (2017), 120-128.

APA	BENDER R, Yucel A, NOYAN V, GÜRPINAR A, Atasoy P, COMU F (2017). The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. GORM:Gynecology Obstetrics & Reproductive Medicine, 23(3), 120 - 128.
Chicago	BENDER R. Ada,Yucel Aykan,NOYAN Volkan,GÜRPINAR Aylin,Atasoy Pınar,COMU Faruk M. The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. GORM:Gynecology Obstetrics & Reproductive Medicine 23, no.3 (2017): 120 - 128.
MLA	BENDER R. Ada,Yucel Aykan,NOYAN Volkan,GÜRPINAR Aylin,Atasoy Pınar,COMU Faruk M. The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. GORM:Gynecology Obstetrics & Reproductive Medicine, vol.23, no.3, 2017, ss.120 - 128.
AMA	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. GORM:Gynecology Obstetrics & Reproductive Medicine. 2017; 23(3): 120 - 128.
Vancouver	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars. GORM:Gynecology Obstetrics & Reproductive Medicine. 2017; 23(3): 120 - 128.
IEEE	BENDER R,Yucel A,NOYAN V,GÜRPINAR A,Atasoy P,COMU F "The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars." GORM:Gynecology Obstetrics & Reproductive Medicine, 23, ss.120 - 128, 2017.
ISNAD	BENDER, R. Ada vd. "The Effect of Adipose Tissue-Derived Mesenchymal Stem Cells on Regeneration of Uterine Scars". GORM:Gynecology Obstetrics & Reproductive Medicine 23/3 (2017), 120-128.