Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells
Yıl: 2019 Cilt: 6 Sayı: 1a Sayfa Aralığı: 213 - 221 Metin Dili: İngilizce DOI: 10.35193/bseufbd.601582 İndeks Tarihi: 09-01-2020
Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells
Öz: In the last decade, particular sphingolipids have become renowned for their participation in cellmembrane functions and signaling proceedings that regulate a wide array of cellular manners. Ceramide is amajor molecule of sphingolipids metabolism. It plays vital roles in mediating the major cellular functions suchas cell differentiation, cell-to-cell communication, apoptosis, and proliferation. Recent studies have shown thatceramide metabolism and their metabolic enzymes take important role during migration and cell mobility process.However, the molecular mechanism of sphingolipids involved is unknown. Our efforts in this study, which are servingmechanistically the understanding of ceramide, will be shade some light on this unknown. In this work, particularly,we looked at the effect of ceramide syntheses on migration ability of UM-SCC-22A head and neck squamouscarcinoma cells. Briefly, we identified that alterations in ceramide metabolism by pharmacological inhibitorfumonisin B1, reducing bioactive ceramides, results in activation of the TGF-ß receptor type I and II (TßRI/II) cellsurface localization and, leading to increased cell migration/invasion in UM-SCC-22A head and neck squamouscarcinoma cells. On the other hand, primary cilia length in UM-SCC-22A cells did not changed by decreasedceramide amount. Thus, these data reveal that ceramide synthases/ceramide is a novel factor that regulates TßRI/II tothe primary cilium, controlling cell migration and cancer metastasis.
Anahtar Kelime: Seramid Metabolizmasının HNSCC Hücrelerinde Primer Silia Uzunluğu ve Tümör Metastazına Etkisi
Öz: Son on yılda, belirli sfingolipitler, hücre zarı fonksiyonlarına katılımları ve çok çeşitli hücresel davranış sistemini düzenleyen sinyalizasyon işlemleriyle önemli hale gelmiştir. Seramid, sfingolipid metabolizmasının ana molekülüdür. Hücre farklılaşması, hücreden hücreye iletişim, apoptoz ve proliferasyon gibi temel hücresel fonksiyonlara aracılık etmede hayati rol oynar. Son zamanlarda yapılan çalışmalar seramid metabolizmasının ve metabolik enzimlerinin hücre göçü ve hücre mobilitesi sürecinde önemli rol oynadığını göstermiştir. Bununla birlikte, söz konusu sfingolipidlerin moleküler mekanizması bilinmemektedir. Mekanik olarak sistemi anlamaya çalışan bu çalışmadaki verilerimiz bu bilinmeyene ışık tutacaktır. Bu çalışmada, özellikle, seramid sentezlerinin UM-SCC-22A baş ve boyun skuamöz karsinom hücrelerinin göç kabiliyeti üzerindeki etkisine baktık. Kısaca, biyoaktif seramidleri azaltan, farmakolojik inhibitör fumonisin B1 ile seramid metabolizmasındaki değişikliklerin, TGF-ß reseptör tip I ve II (TßRI / II) hücre yüzeyine lokalizasyonunun aktifleşmesine ve hücre göçünün / yayılımının artmasına yol açtığını tespit ettik. Öte yandan, UM-SCC-22A hücrelerinde primer silia uzunluğu, seramid miktarında azalma ile değişmedi. Bu nedenle, bu veriler seramid sentezlerinin / seramidin, TßRI / II'yi primer siliuma taşınımını düzenleyen, hücre göçünü ve kanser metastazını kontrol eden yeni bir faktör olduğunu ortaya koymaktadır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Gencer S (2019). Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. , 213 - 221. 10.35193/bseufbd.601582 |
| Chicago | Gencer Salih Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. (2019): 213 - 221. 10.35193/bseufbd.601582 |
| MLA | Gencer Salih Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. , 2019, ss.213 - 221. 10.35193/bseufbd.601582 |
| AMA | Gencer S Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. . 2019; 213 - 221. 10.35193/bseufbd.601582 |
| Vancouver | Gencer S Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. . 2019; 213 - 221. 10.35193/bseufbd.601582 |
| IEEE | Gencer S "Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells." , ss.213 - 221, 2019. 10.35193/bseufbd.601582 |
| ISNAD | Gencer, Salih. "Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells". (2019), 213-221. https://doi.org/10.35193/bseufbd.601582 |
| APA | Gencer S (2019). Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, 6(1a), 213 - 221. 10.35193/bseufbd.601582 |
| Chicago | Gencer Salih Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi 6, no.1a (2019): 213 - 221. 10.35193/bseufbd.601582 |
| MLA | Gencer Salih Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, vol.6, no.1a, 2019, ss.213 - 221. 10.35193/bseufbd.601582 |
| AMA | Gencer S Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi. 2019; 6(1a): 213 - 221. 10.35193/bseufbd.601582 |
| Vancouver | Gencer S Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi. 2019; 6(1a): 213 - 221. 10.35193/bseufbd.601582 |
| IEEE | Gencer S "Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells." Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, 6, ss.213 - 221, 2019. 10.35193/bseufbd.601582 |
| ISNAD | Gencer, Salih. "Impact of the Ceramide Metabolism on Primary Cilia Length and Tumor Metastasis in HNSCC Cells". Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi 6/1a (2019), 213-221. https://doi.org/10.35193/bseufbd.601582 |
