Objective: This study was conducted to determine the factors that affect the job satisfaction and humor styles of nurses and relationship between them. Material and Methods: This descriptive and cross-sectional study was carried out with 681 nurses working in a foundation university hospital and five different provinces of the university. In the study; personal information form, Nurse Job Satisfaction Scale and Humor Styles Scale were used. Data were entered into SPSS 20.0 statistical package program and percentage and frequency distributions, t-test, one-way analysis of variance and correlation analysis were used. Results: The mean score of Nurse Job Satisfaction Scale was 3.58±0.73. Factors such as age, gender, working time in the profession and institution, work position, shift type, participation in scientific activities and following up professional publications affect the job satisfaction of nurses; it was determined that the nurses who are 30 years and younger, male nurses, who have working period of 1-9 years, who work in the service, nurses who have seizures and working at the weekend have low job satisfaction, participate in scientific activities and follow professional publications have high job satisfaction. When the nurses’ humors is examined; the mean scores of nurses used the positive humor styles was found to be higher. It was determined that there was a positive correlation between the use of positive humor style and job satisfaction of nurses. Conclusion: In the study, total job satisfaction levels of nurses were found to be above average. It was determined that the job satisfaction of the nurses using positive humor style was high. Considering the factors affecting the job satisfaction and humor styles of nurses; it may be advisable to provide opportunities for participation in professional development and scientific activities, to make arrangements for working conditions and to plan supportive trainings that will contribute to the development of humor in nurses.