AIM: The study was conducted to determine the occupational professional attitudes and influencing factors of nurses in different generations. METHODS: The study is descriptive. The study included 654 nurses working in health centers in 6 different provinces connected to a foundation university hospital. In this research, personal information form and Professional Attitude Inventory in the Profession (PAIP) was used. The PAIP scores of the nurses are the dependent variable of the study. Age (X and Y generation), gender, marital status, having a child, education status, income level, choosing profession with its own request working time in the profession, unit / title of work, working style, finding adequate education, in-service, internal and external trainings are independent variables. In the analysis of data; descriptive statistics, t test and multiple regression analysis (stepwise) were used. RESULTS: It’s found that the nurses’ PAIP points were 140±15,4 (PAIP max.-min. values:32- 160) It’s determined that 13,9% of the nurses, who attended research, were X generation, 86,1% of them were Y generation; X generation nurses’ average PAIP points (143.9±14.1) were higher than Y generation nurses’ average PAIP points (139.4±15.5) which is statistically meaningful (t= -2.805; p=.006). A significant positive relationship between Y generation nurses’ MPTE scores and gender (being female), having a child, choosing the profession with their own desire, having less than 1 year in the profession, finding sufficient vocational education, participating in internal scientific activities and in-service training (R =.393; R2 =.154; Adjusted R2 =.144; F = 4,805; P (.05).It is found that Y generation nurses attend more to educational and scientific activities that they believe this improves professional behavior. CONCLUSION: It was determined that the mean score of the occupational professional attitude inventory of nurses was above the inventory average. It was determined that the Professional behaviors of the nurses in the X and Y generation differed and the Professional attitude scores of the nurses in the generation X were higher. It was determined that Y generation of nurses had a positive relationship with MPTE scores, especially participation in education and scientific activities. In accordance with this finding; It can be suggested to increase the participation of Y generation nurses in education and scientific activities, and to take into consideration to generational characteristics in humanpower planning.