The presence of some anabolic residues in meat and meat products sold in İstanbul

HAMPIKYAN, Hamparsun; ÇOLAK, Hilal; Aydın, Ali; NAZLI, Bülent

The presence of some anabolic residues in meat and meat products sold in İstanbul

Bülent NAZLI, (İstanbul Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Anabilim Dalı, İstanbul, Türkiye)

Hilal ÇOLAK, (İstanbul Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Anabilim Dalı, İstanbul, Türkiye)

Ali AYDIN, (İstanbul Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Anabilim Dalı, İstanbul, Türkiye)

Hamparsun HAMPIKYAN (İstanbul Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknolojisi Anabilim Dalı, İstanbul, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2005 Cilt: 29 Sayı: 3 Sayfa Aralığı: 691 - 699 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The presence of some anabolic residues in meat and meat products sold in İstanbul

Öz:

Bu çalışmanın amacı, istanbul'da satışa sunulan et ve et ürünlerinde bazı anabolizan madde kalıntılarını (zeranol, trenbolon, dietilstilbestrol, klenbuterol) tespit etmektir. Çalışmada 30 adet et ve 30 adet et ürünü olmak üzere toplam 60 örnek farklı marketlerden toplanarak test materyali olarak kullanıldı. Örneklerdeki anabolik kalıntılar ELISA yöntemi ile tespit edildi. İlk önce geri kazanım testleri yapıldı. Daha sonra örneklerdeki bir veya daha fazla anabolik kalıntılar analiz edildi. Örneklerin tamamında (% 100) zeranol, 48 adedinde (% 80) trenbolon, 21 adedinde (% 35) dietilstilbestrol bulunurken numunelerin hiçbirinde klenbuterol varlığına rastlanmadı. Sonuç olarak; incelenen örneklerin önemli bir kısmında zeranol ve dietilstilbestrol kalıntı miktarları bildirilen kabul edilebilir limitleri aştığı saptandı. Dolayısıyla satışa sunulan et ve et ürünlerinin tüketici sağlığı açısından zararlı olabileceği kanısına varıldı. Bu nedenle Türkiye'de hayvan yetiştirciliğinde anabolik ajanların kullanımı, ciddi olarak kontrol altına alınmalıdır.

Anahtar Kelime: et ürünleri klenbuterol trenbolon gıda bulaşıklılığı İstanbul Türkiye et dietilstibestrol zeranol

Konular: Ziraat Mühendisliği

İstanbul' da satışa sunulan et ve et ürünlerinde bazı anabolizan madde kalıntılarının varlığı üzerine bir çalışma

Öz:

The aim of this study was to detect some anabolic (zeranol, trenboione, diethylstilbestrol, clenbuterol) residues in meat and meat'products marketed in Istanbul. In the study, a total of 60 samples (30 fresh meat and 30 meat products) were obtained from different markets and used as test materials. The anabolic residues in the sample extracts were detected by ELISA. Initially, recovery tests were performed and then residues of one or more anabolics were analyzed in all samples. Among anabolizing residues studied, zeranol was detected in all samples (100%), trenboione was detected in 48 samples (80%) and diethylstilbestrol was detected in 21 samples (35%) whereas no clenbuterol residues were detected in any of the samples. The results indicated that the level of zeranol and diethylstilbestrol in considerable part of samples found to be more than the acceptable limits. Therefore, meat and m,eat products with excessive levels of anabolics might be harmful for the consumer. In conclusion, the use of anabolic agents in animal husbandry in Turkey must be strictly controlled.

Anahtar Kelime: Istanbul Turkey meat diethylstilbestrol zeranol meat products clenbuterol trenbolone food contamination

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Cecave, M.J., Hancock, D.L. : Effects of anabolic steroids on nitrogen metabolism and growth of steers fed corn silage and cornbased diets supplemented with urea or combinations of soybean meal and feathermeal. J. Anim. Sci., 1994; 72: 515- 522.
2. Moran, C, Ourke, J.F., Prendiville, D.J., Bourke, S., Roche, J.F.: The effect of estradiol trenbolonee acetate, or zeranol on growth rate, mammary development, carcass traits, and plasma estradiol concentrations of beef heifers. J. Anim. Sci., 1991; 69: 4249- 4258.
3. Sawaya, W., Lone, K.P., Hasain, A., Dashti, B., Al-Zenki, S.: Screening for estrogenic steroids in sheep and chicken by the application of enzyme-linked immunosorbent assay and a comparison with analysis by gas chromatography-mass spectrometry. Food Chem., 1998; 63: 563-569.
4. Stan, H.J., Abraham, B.: Determination of residues of anabolic drugs in meat by gas chromatography-mass spectrometry. J. Chromatogr., 1980; 195: 231-241.
5. Bauomy, A.L., Neklawy, M., Gergis, A. F.: Oestrogen residues in imported frozen meat. Fleischwirt. Int., 1992; 3: 54-65.
6. Daxenberger, A., Lange, I.G., Meyer, H.H.D.: Detection of anabolic residues in misplaced implant sites in cattle. J. AOAC Int., 2000:83:809-819.
7. Hoffmann, B.: Entwicklung eines Radioimmunotests zur bestimmung von diethylstilbestrol in blutplasma und essbaren Geweben vom Rind. Arch. Lebensmittelhyg., 1980; 31:105-106.
8. Dağoğlu, G., Aksoy, A.: Hayvansal üretimde zeranol. Yüzüncü Yıl Üniv. Sağlık Bilim. Derg., 1995; 1: 103-108.
9. Xiong, Y.L, Moody, W.G., Blanchard, S.P., Liu, G., Burris, W.R. : Postmortem proteolytic and organoleptic changes in hot-boned muscle from grass and grain-fed and zeranol-implanted cattle. Food Res. Int., 1996; 29: 27-34.
10. Egan, C.L., Wilson, L.L.. Drake, T.R., Henning, W.R., Mills, E.W., Meyer, S.D., Kenison, D.C.: Effects of different doses of zeranol on growth, hemoglobin and carcass traits in veal calves. J. Anim. Sci., 1993; 71: 1081-1087.
11. Codex Alimentarius. Food and Nutrition Division. FA0/WH0 Food Standards. Viale delle Terme di Caracalla , Rome, Italy, 1997.
12. European Commission: Unit B3-management of scientific committees II: Opinion of the scientific committee on veterinary measures relating to public health. Assessment of potential risks to human health from hormone residues in bovine meat and meat products. 30 April 1999.
13. Hsu, S., Hsu, H., Eckerlin, R.H., Henion, J.D.: Identification and quantitation of trenbolone in bovine tissue by gas chromatography-mass spectrometry. J. Chromatogr., 1988; 424: 219-229.
14. Laitem, L, Gaspar, P., Bello, I.: Detection of trenbolonee residues in meat and organs of slaughtered animals by thin-layer chromatography. J. Chromatogr., 1978; 147: 538-539.
15. Apple, J.K., Dikeman, M.E., Simms, D.D.: Effects of synthetic hormone implants, singularly or in combinations, on performance carcass traits and longissimus muscle palatability of Holstein steers. J. Anim. Sci., 1991; 69: 4437-4448.
16. Hohls, F.W., Stan, H.J.: Nachweis von trenbolonerückstânden in fleisch durch dünnschichtchromatographie und fluorimetrie. Z. Lebensm. Unters. For., 1978; 167: 252-255.
17. Van Peteghem, C.H., Van Haver, G.M.: Chromatographic purification and radio -immunoassay of diethylstilbestrol residues in meat. Anal. Chim. Acta, 1986; 182: 293-298.
18. Berge, P., Culioli, J., Ouali, A : Performance, muscle composition and meat texture in veal calves administered a beta-agonist (clenbuterol). Meat Sci., 1993; 33: 191-206.
19. Degroodt, J.M., Bukonski, B.W., Beernaert, H., Courtheyn, D.: Clenbuterol residue analysis by HPLC- HPTLC in urine and animal tissues. Z. Lebensm. Unters. For., 1989; 189: 128-131.
20. Smith, D.J.: Stereochemical composition of clenbuterol residues in edible tissues of swine. J. Agr. Food Chem., 2000; 48: 6036- 6043.
21. Delahaut, P., Dubois, M., Pri-bar, I., Buchman, O., Degand, G., Ectors, F.: Development of a specific radioimmunoassay for the detection of clenbuterol residues in treated cattle. Food Addit. Contam., 1991; 8:43-53.
22. Rose, M.D., Shearer, G., Farrington, W.H.H.: The effect of cooking on veterinary drug residues in food: 1.Clenbuterol. Food Addit. Contam., 1995; 12: 67-76.
23. Geesink, G.H., Smulders, F.J.M., Van Laack, H.L.J. M., Van Der Kolk, J.H., Wensing, T.H., Breukink, H.J.: Effects on meat quality of the use of clenbuterol in veal calves. J. Anim. Sci., 1993; 71: 1161-1170.
24. Wilson, R.T., Groneck, J.M., Holland, K.T., Henry, A.C.: Determination of clenbuterol in cattle, sheep and swine tissues by electron ionization gas chromatography/mass spectrometry. J. AOAC Int., 1994; 77: 917-924.
25. Degand, G., Bernes-Duyckaerta, A., Maghuin-Rogister, G.: Determination of clenbuterol tissues and urine by enzyme immunoassay. J. Agr. Food Chem., 1992; 40: 70-75.
26. Brambilla, G., Loizzo, A., Strozzi, M., Fontana, L., Guarino, A., Soprano, V.: Food poisoning following consumption of clenbuterol. J. Am. Med. Assoc, 1997; 278: 635.
27. Meyer, H.H.D., Hoffmann, S.: Development of a sensitive microtitration plate enzyme-immunoassay for the anabolic steroid trenbolone. Food Addit. Contam., 1987; 4: 149-160.
28. Miller, M.F. : Adipose tissue longissimus muscle and anterior pituitary growth and function in clenbuterol-fed heifers. J. Anim. Sci., 1988;66: 12-13.
29. Patel, P.J., Dixon, S.N.: An enzyme immunoassay for the anabolic agent zeranol. Food Addit. Contam., 1989; 6: 91-92.
30. Evrim, M., Güneş, H.: Biometri. İstanbul Üniv. Vet. Fak. Derg., 1994;31: 13-24.
31. Duncan, D.B.: Multiple range and multiple F-tests. Biometrics. 1955; 11: 1-42.
32. Hoffman, B.: Problems of residue and health risks of anabolic agents with hormone like activites. Proceedings of the Scientific Conference on Growth Promotion in Meat Production, European Comm. Dir. Gen. VI. Agric, Official Pub. European Comm., Brussels, 1996:271-296.
33. Jodlbauer, J., Zöllner, P., Lindner, W.: Determination of zeranol, taleranol, zearalenone, alfa and beta zearalenol in urine and tissue by high-performance liquid chromatography-tandem mass spectrometry. Chromatographia, 2000; 51: 681-687.

APA	NAZLI B, ÇOLAK H, Aydın A, HAMPIKYAN H (2005). The presence of some anabolic residues in meat and meat products sold in İstanbul. , 691 - 699.
Chicago	NAZLI Bülent,ÇOLAK Hilal,Aydın Ali,HAMPIKYAN Hamparsun The presence of some anabolic residues in meat and meat products sold in İstanbul. (2005): 691 - 699.
MLA	NAZLI Bülent,ÇOLAK Hilal,Aydın Ali,HAMPIKYAN Hamparsun The presence of some anabolic residues in meat and meat products sold in İstanbul. , 2005, ss.691 - 699.
AMA	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H The presence of some anabolic residues in meat and meat products sold in İstanbul. . 2005; 691 - 699.
Vancouver	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H The presence of some anabolic residues in meat and meat products sold in İstanbul. . 2005; 691 - 699.
IEEE	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H "The presence of some anabolic residues in meat and meat products sold in İstanbul." , ss.691 - 699, 2005.
ISNAD	NAZLI, Bülent vd. "The presence of some anabolic residues in meat and meat products sold in İstanbul". (2005), 691-699.

APA	NAZLI B, ÇOLAK H, Aydın A, HAMPIKYAN H (2005). The presence of some anabolic residues in meat and meat products sold in İstanbul. Turkish Journal of Veterinary and Animal Sciences, 29(3), 691 - 699.
Chicago	NAZLI Bülent,ÇOLAK Hilal,Aydın Ali,HAMPIKYAN Hamparsun The presence of some anabolic residues in meat and meat products sold in İstanbul. Turkish Journal of Veterinary and Animal Sciences 29, no.3 (2005): 691 - 699.
MLA	NAZLI Bülent,ÇOLAK Hilal,Aydın Ali,HAMPIKYAN Hamparsun The presence of some anabolic residues in meat and meat products sold in İstanbul. Turkish Journal of Veterinary and Animal Sciences, vol.29, no.3, 2005, ss.691 - 699.
AMA	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H The presence of some anabolic residues in meat and meat products sold in İstanbul. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(3): 691 - 699.
Vancouver	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H The presence of some anabolic residues in meat and meat products sold in İstanbul. Turkish Journal of Veterinary and Animal Sciences. 2005; 29(3): 691 - 699.
IEEE	NAZLI B,ÇOLAK H,Aydın A,HAMPIKYAN H "The presence of some anabolic residues in meat and meat products sold in İstanbul." Turkish Journal of Veterinary and Animal Sciences, 29, ss.691 - 699, 2005.
ISNAD	NAZLI, Bülent vd. "The presence of some anabolic residues in meat and meat products sold in İstanbul". Turkish Journal of Veterinary and Animal Sciences 29/3 (2005), 691-699.