Yıl: 2005 Cilt: 4 Sayı: 2 Sayfa Aralığı: 107 - 111 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67

Öz:
Bu çalışmada, normal prostat dokusu, benign prostat hiperplazisi (BPH), prostatik intraepitelyal neoplazi (PIN) ve prostat kanseri doku örneklerinde proliferasyon aktivitesi immünohistokimyasal yöntemle Ki-67 monoklonal antikoru kullanılarak değerlendirildi. Ki-67, hücre siklusu boyunca eksprese edilen nükleer bir non-histon proteindir. Proliferasyon oranı, normal prostat glanduler epitelinde % 0.3-0.8 olarak, BPH de % 0.5-2.2, yüksek evreli PIN de % 1.6-3.7 ve prostat kanser vakalarında % 5-30 olarak bulundu. Proliferasyon aktiviteleri normal prostat dokusu ile karşılaştırıldığında BPH de hafif artış, PIN ve prostat kanserinde anlamlı yüksek artış gösterdi. Ki-67 proliferasyonndeksinin kanser grubunda Gleason sınıflandırmasıyla ilişkili olduğu bulundu. Ki-67 proliferasyon indeksinin değerli bir tam markın olarak PIN ve tümöral dokularda kullanılabileceği sonucuna varıldı.
Anahtar Kelime: prostat neoplazma monoklonal antikorlar immünohistokimya

Konular: Biyokimya ve Moleküler Biyoloji

Normal ve karsinomatöz prostat dokularının çoğalma aktivitelerinin Ki-67 ile değerlendirilmesi

Öz:
In this study proliferation activities of normal prostate tissue, benign prostate hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN) and prostate cancer samples were evaluated immunohistochemically by using the monoclonal anti-Ki-67 antibody. Ki-67 is a nuclear non-histone protein expressed throughout the cell cycle. The proliferation rate was found to be 0.3-0.8 % in normal prostate glandular epithelium, 0.5-2.2 % in BPH, 1.6-3.7 % in high grade PIN and 5-30 % in prostate cancer cases. BPH showed a slightly higher, whereas PIN and prostate cancer showed significantly higher proliferation activity compared to normal prostate tissue. The Ki-67 proliferation index was found to correlate well with the Gleason classification in the cancer group. It is concluded that the Ki-67 proliferation index can be used a valuable diagnostic marker in PIN and tumor tissue.
Anahtar Kelime: monoclonal antibodies immunohistochemistry prostate neoplasms

Konular: Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Bantis A, Giannopoulos A, Gonidi M, Liossi A, Aggelonidou E, Petrakakou E, Athanassiades P, and Athanassiadou P. Expression of p120, Ki- 67 and PCNA as proliferation biomarkers in imprint smears of prostate carcinoma and their prognostic value. Cytopathology. 15: 25-31, 2004.
  • Brown DC, Cole D, Gatter KC, and Mason DY. Carcinoma of the cervix uteri: an assessment of tumour proliferation using the monoclonal antibody Ki-67. Br J Cancer. 57: 178-181, 1988.
  • Brown DC, Gatter KC. Monoclonal antibody Ki-67: Its use in histopathology. Histopathology. 7: 489-503, 1990.
  • Bubendorf L, Sauter G, Moch H, Schmid HP, Gasser TC, Jordan P, and Mihatsch MJ. Ki67 labelling index: An independent predictor of progression in prostate cancer treated by radical prostatectomy. J Pathol. 178: 437-441, 1996.
  • Bubendorf L, Tapia C, Gasser TC, Casella R, Grunder B, Moch H, Mihatsch MJ, and Sauter G. Ki67 labelling index in core needle biopsies independently predicts tumor-specific survival in prostate cancer. Hum Pathol. 29: 949-954, 1998.
  • Claus S, Wrenger M, Senge T, and Schulze H. Immunohistochemical determination of age related proliferation rates in normal and benign hyperplastic human prostates. Urol Res. 21: 305-308, 1993.
  • Colombel M, Vacherot F, Diez SG, Fontaine E, Buttyan R, and Chopin D. Zonal variation of apoptosis and proliferation in the normal prostate and in benign prostatic hyperplasia. Br J Urol. 82: 380-385, 1998.
  • Cowen D, Troncoso P, Khoo VS, Zagars GK, Von Eschenbach AC, Meistrich ML, and Pollack A. Ki-67 staining is an independent correlate of Biochemical Failure in prostate cancer treated with radiotherapy. Clin Cancer Res. 8: 1148-1154, 2002.
  • Feneley MR, Young MPA, Chinyema C, Kirby RS, and Parkinson MC. Ki-67 expression in early prostate cancer and associated pathological lesions. J Clin Pathol. 49: 741-748, 1996.
  • Gallee MPW, Jong EV, Kate F JNT, Schroeder FH, and Van der Kwast TH. Monoclonal antibody Ki-67 defined growth fraction in benign prostatic hyperplasia and prostatic cancer. J Urol. 142: 1342-1346, 1989.
  • Gerdes J, Lemke H, Baisch H, Wacker HH, Schwab U, and Stein H. Cell cycle analysis of a cell proliferationassociated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 133: 1710-1715, 1984.
  • Gerdes J. An immunohistological method for estimating cell growth fractions in rapid histopathological diagnosis during surgery. Int J Cancer. 35: 169-171, 1985.
  • Gerdes J, Becker MH, Key G, and Cattoretti G. Immunohistological detection of tumour growth fraction (Ki-67 antigen) in formalin-fixed and routinely processed tissues. J Pathol. 168: 85-87, 1992.
  • Hall PA, Levison DA. Assessment of cell proliferation in histologic material. J Clin Pathol. 43: 184-192, 1990.
  • Hattori H. Sclerosing haemangioma of the lung is positive for MIB-1 in cell membrane and cytoplasmic staining pattern. Histopathology. 40: 291-293, 2002.
  • Jemal A, Tiwari RC., Murray T, Ghafoor A, Samuels A, Ward E,. Feuer EJ, and Thun MJ. Cancer Statistics, 2004. CA Cancer J Clin. 54: 8 – 29, 2004.
  • Key G, Becker MHG, Baron B, Duchrow M, Schluter C, Flad HD, and Gerdes J. New Ki-67 equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67 epitope. Lab Invest. 68: 629-636,1993.
  • Leong AS, Wright J. The contribution of immunohistochemical staining in tumor diagnosis. Histopathology. 11: 1295-1305, 1987.
  • Mc Loughlin J, Foster CS, Price P, Williams G, and Abel PD. Evaluation of Ki-67 monoclonal antibody as prognostic indicator for prostatic carcinoma. Br J Urol. 72: 92-97, 1993.
  • Meyer JS, Sufrin G, Martin SA. Proliferative activity of benign human prostate, prostatic adenocarcinoma and seminal vesicle evaluated by thymidine labeling. J Urol. 128: 1353-1356, 1982.
  • Oxley JD, Abbott CD, Gillatt DA, and MacIver AG. Ductal carcinomas of the prostate: a clinicopathological and immunohistochemical study. Br J Urol. 81: 109-115,1998.
  • Sasaki K, Murakami T, Kawasaki M, and Takahashi M. The cell cycle associated change of the Ki-67 reactive nuclear antigen expression. J Cell Physiol. 133: 579-584, 1987.
  • Schonk DM, Kuijpers HJH, Van Drunen E, Van Dalen CH, Van Kessel AHG, Verheijen R, and Ramaekers FC. Assignment of the gene(s) involved in the exspression of the proliferation-related Ki-67 antigen to human chromosome 10. Hum Genet. 83: 297-299, 1989.
  • Shi SR, Key ME, Kalra KL. Antigen retrieval in formalinfixed, paraffin-embedded tissues: An enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem. 39: 741-747, 1991.
  • Stattin P, Damber J, Karlberg L, Tavelin B, and Damber JE. Cell proliferation assessed by Ki-67 immunoreactivity on formalin fixed tissues is a predictive factor for survival in prostate cancer. J Urol. 157: 219-222, 1997.
  • Tamboli P, Amin MB, Schultz DS, Linden MD, and Kubus J. Comparative analysis of the nuclear proliferative index (Ki-67) In benign prostate, prostatic intraepithelial neoplasia, and prostatic carcinoma. Mod Pathol. 9: 1015-1019, 1996.
APA KOYUTURK M, KILIÇARSLAN I, AYTEKİN Y (2005). Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. , 107 - 111.
Chicago KOYUTURK Meral,KILIÇARSLAN Işın,AYTEKİN Yener Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. (2005): 107 - 111.
MLA KOYUTURK Meral,KILIÇARSLAN Işın,AYTEKİN Yener Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. , 2005, ss.107 - 111.
AMA KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. . 2005; 107 - 111.
Vancouver KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. . 2005; 107 - 111.
IEEE KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y "Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67." , ss.107 - 111, 2005.
ISNAD KOYUTURK, Meral vd. "Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67". (2005), 107-111.
APA KOYUTURK M, KILIÇARSLAN I, AYTEKİN Y (2005). Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. Journal of Cell and Molecular Biology, 4(2), 107 - 111.
Chicago KOYUTURK Meral,KILIÇARSLAN Işın,AYTEKİN Yener Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. Journal of Cell and Molecular Biology 4, no.2 (2005): 107 - 111.
MLA KOYUTURK Meral,KILIÇARSLAN Işın,AYTEKİN Yener Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. Journal of Cell and Molecular Biology, vol.4, no.2, 2005, ss.107 - 111.
AMA KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. Journal of Cell and Molecular Biology. 2005; 4(2): 107 - 111.
Vancouver KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67. Journal of Cell and Molecular Biology. 2005; 4(2): 107 - 111.
IEEE KOYUTURK M,KILIÇARSLAN I,AYTEKİN Y "Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67." Journal of Cell and Molecular Biology, 4, ss.107 - 111, 2005.
ISNAD KOYUTURK, Meral vd. "Evaluation of the proliferating activities of normal and carcinomatous prostatic tissues with Ki-67". Journal of Cell and Molecular Biology 4/2 (2005), 107-111.