Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone

ŞAHİN, Nazan; Temoçin, Zülfikar; LALE, Mustafa

Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone

Mustafa LALE, (Türkiye Odalar ve Borsalar Birliği, Ankara, Türkiye)

Nazan ŞAHİN, (Kırıkkale Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Kırıkkale, Türkiye)

Zülfikar TEMOÇİN (Kırıkkale Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Kırıkkale, Türkiye)

Gazi Üniversitesi Fen Bilimleri Dergisi

1 0

Yıl: 2005 Cilt: 18 Sayı: 3 Sayfa Aralığı: 365 - 373 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone

Öz:

Bu çalışmada kolon yöntemi kullanılarak $Fe^{2+}$ iyonlarının Saccharomyces cerevisiae immobilize edilmiş ponza taşına adsorpsiyon şartları araştırıldı. Bu amaçla; adsorpsiyona çözelti pH sının, iyon başlangıç derişiminin ve adsorplanan iyonların geri kazanılması için uygun çözelti türü ve derişiminin etkisi araştırıldı. Maksimum adsorpsiyonun sağlandığı pH değerinin 5 olduğu belirlendi. Optimum pH değerinde, adsorbanın metal tutma kapasitesi 60 $mu g/mL$ başlangıç derişiminde 1,81mg/g dır. Desorpsiyona HC1, NaCl, NH4C1 ve EDTA çözeltilerinin etkisi araştırıldı. En uygun çözeltinin 0,2 M'lık EDTA çözeltisi olduğu gözlendi. 0,2 M EDTA çözeltisi ile %98 oranında desorpsiyon sağlandı.

Anahtar Kelime:

$Fe^{2+}$ iyonlarının Saccharomyces cerevisiae immobilize edilmiş ponza taşı ile adsorpsiyonu

Öz:

In this study, the adsorption conditions of $Fe^{2+}$ ions on Saccharomyces cerevisae immobilized pumice stone were investigated by column method. In order to perform this aim, the effect of solution pH value, initial concentration of ions and suitable solution and concentration for recovery of adsorbed ions were investigated. At pH 5, the maximum adsorption was obtained. At optimal pH value, adsorption capacity of sorbent for metal was obtained as 1,81 $mg.g^{-1}$, when initial concentration was 60 $mg.ml^{-1}$. Influence of HC1, NaCl, NH4Cl and EDTA solutions on desorption were investigated and maximum recovery was achieved by 0,2 M EDTA solution. Desorption were 98% level provided by 0,2 M EDTA solution.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1.Madrid, Y. and Camara , C., "Biological Substrates for Metal and Preconcentration and Speciation", Trends in Anal. Chem., 16, 36-44 (1997).
2.Drake L.R., and Rayson G.D., "Plant-Derived Materials for Metal Ion-Selective Binding and Preconcentration".Anal. Chem., 1, 22-27 (1996).
3.Aksu. Z. and Kutsal, T., "Bioseparation Process for Removing Lead(II) Ions from Waste Water by Using C.vulgaris", J. Chem. Tech. Biotech., 52, 109-118 (1991).
4.Tsezos M., Baird H.I. and Shemilt, L.W., "The Kinetics of Radium Biosorption", The Chem. Eng. J., 33, B35-B41(1986).
5.Crist, R.H., Oberhaser, K. Shank, N., Nguyen, M., "Nature of Binding between Metallic Ions and Algal Cell Walls",Environ. Set Tech., 15, 1212-1217 (1981).
6.Mahan, C.A. and Holcombe, J.A., "Immobilization of Algae Cells on Silica Gel and Their Characterization for Trace Metal Preconcentration", Anal. Chem., 64, 1933-1940 (1992).
7.Bağ, H., Türker, A.R. and Lale, M., "Determination of Cu, Zn, Fe, Ni and Cd by Flame Atomic Absorption Spectrophotometry after Preconcentration by Escherichia coli Immobilized on Sepiolite", Talanta, 51, 1035-1043 (2000)
8.Maquieria, A., Elmahadi, H.A.M. and Puchades, R., "Use of Saccharomyces cerevisiae in Flow Injection Atomic Absorption Spectrometry for Trace Metal Preconcentration", Anal, Chem., 66, 1462-1467 (1994).
9.Gerin, P.A., Asther, M. and Rouxhet, P.G., "Peroxidase Production by the Filamentous Fungus Phanerochaete chrysosporium in Relation to Immobilization in "Filtering" Carriers", Enzyme and Microbial Tech., 20, 294-300 (1997)
10.Garnham, G.W., Codd, G.A. and Gadd, G.M., "Accumulation of Cobalt, Zinc and Manganese by the Estuarine Green Microalga Chlorella salina Immobilized in Alginate Microbeads", Environ. Set Tech., 26 (9), 1764-1770 (1992)
11.Elmahadi, H.A.M. Greenway, G.M., "Immobilization of Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry", J. Anal. At. Spect., 6, 643-651 (1991).
12.Maquieria, A., Elmahadi, H.A.M. and Puchades, R., "Technique and Support for Microorganism Immobilization Application to Trace Metals Enrichment by Flow Injection Atomic Absorption Spectrometry", Analyst, 121, 1633-1640(1996).
13.Mahan, C.A. and Holcombe, J.A., "Preconcentration of Trace Metals Using Silica-Immobilized Algae Cells in a Chromatographic Separation Procedure", Spectrochimica Acta, 47B, 1483-1490 (1992).
14.Madrid, Y., Cabrera, C., Corona, T. P., and Camara, C., "Speciation of Methyl Mercury and Hg(II) Using Saccharomyces cerevisiae, Determination by Continuous Flow Mercury Cold Vapor Generation Atomic Absorption Spectrometry", Anal. Chem., 67, 750-754, (1995)
15.Suh, J.H., Yun, J.W. and Kim, D.S., "Comparison of Pb2+ Accumulation Characteristics Between Live and Dead Cells of Saccharomyces cerevisiae and Aureobasidium pullulans", Biotechnology Letters, 20 (3), 247-251, (1998)
16.Bassari, A., Akyüz, T. and Kurtcebe, T., "The Removal of Th, Cs and Sr Ions from Solutions Using Granulated Pumice Stone", J. of Inclusion Phenomena and Molecular Recognition in Chemistry, 26, 83-88, (1996)
17.Bruce M.M., "Ponza Pazarı", M.T.A. Genel Müdürlüğü Fizibilite Etüdleri Dairesi, Ankara (1991).
18.Bağ, H., Lale, M. and Türker, A. R., "Determination of Iron and Nickel by Flame Atomic Absorption Spectrophotometry after Preconcentration on Saccharomyces cerevisiae Immobilized Sepiolite", Talanta, 47, 689-697 (1998).
19.Skoog, D.A. and West, D.M., "Fundamentals of Analytical Chemistry 3nd ed." Holt Rinehart and Winston, USA,790-791(1976).
20.Elmahadi, H.A.M. Greanway, G.M., "Immobilization Algae as a Reagent for Preconcentration in Trace Element Atomic Absorption Spectrometry", J. Anal. A. Spectrum., 6, 643-651 (1991).
21.Robles, L.C. and Aller, A.J., "Preconcentration of Beryllium on the Outer Membrane of Esherichia coli and Pseudomonas putida Prior to Determination by Electrothermal Atomic Absorption Spectrometry", J. Anal. A. Spectrum., 9, 871-878 (1994)
22.Shengjun, M. and Holcombe, J.A., "Preconcentration of Copper on Algae and Determination by Slurry Graphite Furnace Atomic Absorption Spectrometry", Anal. Chem., 62, 1994-1997 (1990)
23.Tsezos, M., Baird, M.H.I and Shemilt, L.W., "The Elution of Radium Adsorbed by Microbial Biomass", The Chem. Eng. J., 34, B57-B62 (1987)
24.Nakajima, A., and Sakagucki,T., "Selective Accumulation of Heavy Metals by Microorganisms", App. Microbiology and Biotech., 24, 59-65 (1986)
25.Tsezos, M., "Recovery of Uranium from Biological Adsorbents-Desorption Equilibrium", Biotech, and Bioeng., 26, 973-981 (1984)
26.Lale, M., Temoçin, Z. and Bağ, H., "Sorption Behavior of Copper(II), Zinc(II) and Nickel(II) on Formaldehyde Cross-Linked Saccharomyces cerevisiae Immobilized on Pumice Stone " Fresenius Environmental Bulletin, 10, 736-740 (2001)

APA	LALE M, ŞAHİN N, Temoçin Z (2005). Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. , 365 - 373.
Chicago	LALE Mustafa,ŞAHİN Nazan,Temoçin Zülfikar Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. (2005): 365 - 373.
MLA	LALE Mustafa,ŞAHİN Nazan,Temoçin Zülfikar Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. , 2005, ss.365 - 373.
AMA	LALE M,ŞAHİN N,Temoçin Z Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. . 2005; 365 - 373.
Vancouver	LALE M,ŞAHİN N,Temoçin Z Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. . 2005; 365 - 373.
IEEE	LALE M,ŞAHİN N,Temoçin Z "Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone." , ss.365 - 373, 2005.
ISNAD	LALE, Mustafa vd. "Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone". (2005), 365-373.

APA	LALE M, ŞAHİN N, Temoçin Z (2005). Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. Gazi Üniversitesi Fen Bilimleri Dergisi, 18(3), 365 - 373.
Chicago	LALE Mustafa,ŞAHİN Nazan,Temoçin Zülfikar Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. Gazi Üniversitesi Fen Bilimleri Dergisi 18, no.3 (2005): 365 - 373.
MLA	LALE Mustafa,ŞAHİN Nazan,Temoçin Zülfikar Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. Gazi Üniversitesi Fen Bilimleri Dergisi, vol.18, no.3, 2005, ss.365 - 373.
AMA	LALE M,ŞAHİN N,Temoçin Z Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. Gazi Üniversitesi Fen Bilimleri Dergisi. 2005; 18(3): 365 - 373.
Vancouver	LALE M,ŞAHİN N,Temoçin Z Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone. Gazi Üniversitesi Fen Bilimleri Dergisi. 2005; 18(3): 365 - 373.
IEEE	LALE M,ŞAHİN N,Temoçin Z "Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone." Gazi Üniversitesi Fen Bilimleri Dergisi, 18, ss.365 - 373, 2005.
ISNAD	LALE, Mustafa vd. "Adsorption of $Fe^{2+}$ IONS with Saccharomyces cerevisiae immobilized pumice stone". Gazi Üniversitesi Fen Bilimleri Dergisi 18/3 (2005), 365-373.