Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele

BAŞAR, M. Murad; ÇAĞLAYAN, Osman; Kisa, Ucler; FERHAT, Mehmet

Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele

Üçler KISA, (Kırıkkale Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

M. Murad BAŞAR, (Kırıkkale Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Kırıkkale, Türkiye)

Mehmet FERHAT, (Kırıkkale Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, Kırıkkale, Türkiye)

Osman ÇAĞLAYAN (Kırıkkale Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kırıkkale, Türkiye)

Turkish Journal of Medical Sciences

0 0

Yıl: 2008 Cilt: 38 Sayı: 2 Sayfa Aralığı: 105 - 110 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele

Öz:

Amaç: Bu çalışmanın amacı varikoselli infertil hastalar ile normal fertil olgular arasında seminal plazma EGF ve TGF-p düzeylerini karşılaştırmak, ve seminal plazma EGF ve TGF-P düzeyleri, semen parametreleri ve serum hormon düzeyleri arasındaki ilişkiyi incelemektir. Materyal ve Metot: Varikoselli 100 infertil hasta (Grup-1) çalışmaya alındı. İki ile 5 günlük cinsel perhiz sonrası semen analizi uygulandı. Semen analiz sonuçlarına göre bu hastalar oligoastenoeratozoospermisi olanlar (Grup-1) ve normal semen analizi olanlar (Grup-2) olarak iki gruba ayrıldı. İlave olarak, skrotal patolojisi olmayan 100 fertil olgu kontrol grubu (Grup-3) olarak kabul edildi ve semen analizi bunlarda da uygulandı. Seminal plazma EGF ve TGF-p düzeyleri ELİZA yöntemi ile ölçüldü. Ayrıca, tüm olgularda serum gonadotropin ve seks steroid düzeyleri ölçüldü, istatistiskel analiz tek yönlü ANOVA test ve post hoc Bonferoni test ile yapıldı. Bulgular: Her iki grup arasında semen analizi parametreleri bakımından anlamlı farklık vardı (P < 0,05). Seminal plazma EGF ve TGF-p düzeyleri varikoselli olgularda (Grup-1 ve Grup-2) Grup-3'den daha yüksekti. Ancak, istatistiksel fark sadece TGF-p düzeylerinde saptandı (P = 0,017). Seminal plazma EGF ve TGF-P seminal parametreler ile negatik ilişki göstermekteydi (P < 0,05). Bununa karşın, bu büyüme faktörleri ile serum hormon düzeyleri arasında ilişki, yoktu. Sonuç: Bu çalışma göstermektedir ki, seminal plasma EGF ve TGF-P düzeylerindeki artış varikoselli hastalarda seminal parametrelerde azalma ile birliktedir. Bununla birilkte, etki TGF-p ile daha belirgindir.

Anahtar Kelime: Sitokinler Varikosel Dönüştürücü büyüme faktörü beta Epidermal büyüme faktörü Kısırlık

Konular: Cerrahi

Varikoselli hastalarda seminal plazma transforme edici büyüme faktörü-B (TGF-B) ve epidermal büyüme faktörü (EGF) düzeyleri

Öz:

Aims: The aim of this study was to analyze seminal plasma epidermal growth factor (EGF) and transforming growth factor-B (TGF-J3) levels in infertile patients with varicocele and normal fertile cases, and to evaluate the relationships between seminal plasma EGF and TGF-p, seminal parameters and serum hormone levels. Materials and Methods: A total of 100 patients with varicocele were enrolled into the study. Semen analyses were performed after 2 and 5 days of sexual abstinence. These patients were divided into two groups based on the results of semen analysis as oligoasthenoteratozoospermia (Group 1) and normal semen analysis (Group 2). Additionally, 100 fertile cases without any scrotal pathology were accepted as the control group (Group 3), and semen analysis was also performed in these cases. Seminal plasma EGF and TGF-P levels were measured with ELISA. Additionally, serum gonadotropin and serum sex steroid levels were measured in patients and controls. Statistical analysis was performed by using one-way ANOVA test and post hoc •Bonferroni test. Results: There was a statistically significant difference between patients and controls regarding seminal parameters (P < 0.05). Seminal plasma EGF and TGF-p levels were higher in patients with varicocele (Group 1 and Group 2) than Group 3; however, there was a statistically significant difference only in TGF-P levels (P = 0.017). Seminal plasma EGF and TGF-p levels showed a negative relationship with seminal parameters (P < 0.005). However, there was no relationship between these growth factors and serum hormone levels. Conclusions: This study showed that increased levels of EGF and TGF-p' were associated with decreased seminal parameters in patients with varicocele. However, the relationship was more evident with TGF-p.

Anahtar Kelime: Infertility Cytokines Varicocele Transforming Growth Factor beta Epidermal Growth Factor

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Hendry WF, Sommerville IF, Hall RR, Pugh RC. Investigation and treatment of the subfertile male. Br J Urol 1973; 45: 684-92.
2. Cockett AT, Takihara M, Cosentio MJ. The varicocele. Fertil Steril 1984:41:5-11.
3. Kursh ED. What is the incidence of varicocele in a fertile population? Fertil Steril 1987; 48: 510-1.
4. Hargreave TB. Varicocele - a clinical enigma. Br J Urol 1993; 72: 401-8.
5. Nagler HM, Luntz RK, Martinis FG. Varicocele. In: Lipshultz LI, Howards SS, editors. Infertility in the Male. St. Louis: Mosby; 1997. pp. 336-59.
6. Sigman M, Jarow JP. Male infertility. In: Walsh PC, Retik AB, Vaughan ED Jr, Wein AJ, editors. Capmbells's Urology. Philadelphia: Saunders; 2002. pp: 1475-531.
7. Roser JF. Endocrine and paracrine control of sperm production in stallions. Anim Reprod Sci 2001; 68: 139-51.
8. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 2004; 6: 259-68.
9. Hirata Y, Uchihashi M, Hazama M, Fujita T. Epidermal growth factor in human seminal plasma. Horm Metab Res 1987; 19:35-7.
10. Adekunle AO, Falase EA, Ausmanus M, Kopf GS, Van-Arsdalen KN, Teuscher C. Comparative analysis of blood plasma epidermal growth factor concentrations, hormonal profiles and semen parameters of fertile and infertile males. Afr J Med Sci 2000; 29: 123-6.
11. Lui WY, Lee WM, Cheng CY. TGF-ps: their role in testicular function and Sertoli cell tight junction dynamics. Int J Androl 2003; 26: 147-60.
12. Benahmed M, SordoilletC, Chauvin MA, de Peretti E, Morera AM. On. the mechanisms involved in the inhibitory and stimulatory actions of transforming growth factor-p on porcine testicular steroidogenesis: an in vitro study. Mol Cell Endocrinol 1989; 67: 155-64.
13. Fuse H, Okumura M, Sakamoto M, Kazama T, Katayama T. Epidermal growth factor in seminal plasma from patients with hypogonadism changes after hormone replacement. Urol Int 1994:52:9-13.
14. Rowe PJ, Comhaire FH, Hargreave TB, Mahmoud AMA. WHO manual for the standardized investigation, diagnosis and management of the infertile male. Cambrige (UK): Bath Press;2000.
15. Kruger TF, Du Troit TC, Franken DR, Acosta AA, Oehninger SC, Menkveld R et al. A new computerized method of reading sperm morphology (strict criteria) is as efficient as technician reading. Fertil Steril 1993:59:202-9.
16. Avallet O, Vigier M, Leduque P, Dubois PM, Saez JM. Expression and regulation of transforming growth factor-beta 1 messenger ribonucleic acid and protein in cultured porcine Leydig and Sertoli cells. Endocrinology 1987; 134: 2079-87.
17. Khan SA, Mirsafian M, Howdeshell K, Dorrington JJ. Transforming growth factor-B inhibits DNA synthesis in immature rat Leydig cells in vitro. Mol Cell Endocrinol 1999; 148: 21-8.
18. Morerra AM, Esposito G, Ghiglieri C, Chauvin MA, Hartmann DJ, Benahmed M. Transforming growth factor-pi inhibits gonadotropin action in cultured porcine Sertoli cells. Endocrinology 1988; 130: 831-6.
19. Gnessi L, Fabbri A, Spera G. Gonadal peptides as mediators of development and functional control of the testis: an integrated system with hormones and local environment. Endocr Rev 1997; 18: 541-609.
20. Prepin J, Le Vigouroux P. Inhibitions by TGF-B1 of the in vitro thymulin-stimulated proliferation of gonocytes from fetal rat testes. Reprod Nutrition Develop 1997; 37: 203-6.
21. Olaso R, Pairault C, Boulogne B, Durand P, Habert R. Transforming growth factor-pi and P2 reduce the number of gonocytes by increasing apotosis. Endocrinology 1998; 139: 733-40.
22. Loras B, VĞtele F, El-Malki A, Rollet J, Soufir JC, Benahmed M. Seminal transforming growth factor-p normal and infertile men. Hum Reprod 1999; 14: 1534-9.
23. Dobashi M, Fujisawa M, Yamazaki T, Okada H, Kamidono S. Distribution of intracellular and extracellular expression of transforming growth factor-beta 1 (TGF-betal) in human testis and their association with spermatogenesis. Asian J Androl 2002; 4:105-9.
24. Salama N, Tsuji M, Tamura M, Kagawa S. Transforming growth factor (pi) in testes of aged and diabetic rats: correlation with testicular function. Arch Androl 2001; 47: 217-26.
25. Edwin F, Wiepz GJ, Singh R, Peet CR, Chaturvedi D, Bertics PJ et al. A historical perspective of the EGF receptor and related systems. Methods Mol Biol 2006; 327: 1-24.
26. Richards RC, Lewis-Jones DI, Walker JM, Desmond AD. Epidermal growth factor (urogastrone) in human seminal plasma from fertile and infertile males. Fertil Steril 1988; 50: 640-3.
27. Orsini B, Brocchi A, Calabro A, Fedi P, Tommasi MS, Surrenti C. Radioimmunoassay of epidermal growth factor in human saliva and gastric juice. Clin Biochem 1991; 24: 135-41.
28. D'Cruz OJ, Haas GG Jr. Immunoreactive human epidermal growth factor in human seminal plasma. J Clin Endocrinol Metab 1989; 68:1136-40.
29. Delgado SR, Ramirez K, Mallea E, Epidermal growth factor (EGF) is not an index of seminiferous tubular function. Andrologia 1991:23:241-3.
30. Naz RK, Kaplan P. Effects of epidermal growth factor on human sperm cell function. J Androl 1993; 14: 240-7.
31. Cheng D, Zheng XM, Li SW, Yang ZW, Hu LQ. Effects of epidermal growth factor on sperm content and motiiity of rats with surgically induced varicoceles. Asian J Androl 2006; 8: 713-7.

APA	Kisa U, BAŞAR M, FERHAT M, ÇAĞLAYAN O (2008). Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. , 105 - 110.
Chicago	Kisa Ucler,BAŞAR M. Murad,FERHAT Mehmet,ÇAĞLAYAN Osman Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. (2008): 105 - 110.
MLA	Kisa Ucler,BAŞAR M. Murad,FERHAT Mehmet,ÇAĞLAYAN Osman Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. , 2008, ss.105 - 110.
AMA	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. . 2008; 105 - 110.
Vancouver	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. . 2008; 105 - 110.
IEEE	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O "Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele." , ss.105 - 110, 2008.
ISNAD	Kisa, Ucler vd. "Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele". (2008), 105-110.

APA	Kisa U, BAŞAR M, FERHAT M, ÇAĞLAYAN O (2008). Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. Turkish Journal of Medical Sciences, 38(2), 105 - 110.
Chicago	Kisa Ucler,BAŞAR M. Murad,FERHAT Mehmet,ÇAĞLAYAN Osman Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. Turkish Journal of Medical Sciences 38, no.2 (2008): 105 - 110.
MLA	Kisa Ucler,BAŞAR M. Murad,FERHAT Mehmet,ÇAĞLAYAN Osman Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. Turkish Journal of Medical Sciences, vol.38, no.2, 2008, ss.105 - 110.
AMA	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. Turkish Journal of Medical Sciences. 2008; 38(2): 105 - 110.
Vancouver	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele. Turkish Journal of Medical Sciences. 2008; 38(2): 105 - 110.
IEEE	Kisa U,BAŞAR M,FERHAT M,ÇAĞLAYAN O "Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele." Turkish Journal of Medical Sciences, 38, ss.105 - 110, 2008.
ISNAD	Kisa, Ucler vd. "Seminal plasma transforming growth factor- B (TGF- B) and epidermal growth factor (EGF) levels in patients with varicocele". Turkish Journal of Medical Sciences 38/2 (2008), 105-110.