Sreeparna BANERJEE
(Orta Doğu Teknik Üniversitesi, Ankara, Türkiye)
Proje Grubu: TÜBİTAK SBAG ProjeSayfa Sayısı: 0Proje No: 114S937Proje Bitiş Tarihi: 15.12.2015Türkçe

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C/EBPβ’nın Caco-2 Hücre Farklılaşması Sürecinde İndüklenen Otofajideki Rolünün Anlaşılması
Bağırsak epitel hücrelerinin dengeli ve sürekli üretimi ile ilgili moleküler mekanizmalar tam olarak aydınlatılmış değildir. Karmaşık bir süreç olan farklılaşma, çeşitli hücre dışı ve içi sinyaller, sinyal yolakları ve bu sinyal-sinyal yolakları tarafından aktive veya inhibe edilen transkripsiyon faktörleri tarafından düzenlenmektedir. Hücre içi Ca2+ seviyesindeki değişim, glikoz veya amino asit yoksunluğu gibi düzensizlikler, endoplazmik retikulum (ER) stres yanıtına yol açar. Bilinmektedir ki, ER stresi ve otofaji yolakları belirli koşullarda bir arada etkilidir. Yaptığımız çalışmalarda konfluensiye ulaştıktan sonra, enterosit benzeri hücrelere farklılaşabilen kolon kanser hücre hattı Caco-2’de, farklılaşma sürecinde hem ER stresi hem de otofaji gözlenmiştir. Bu proje kapsamında ER stresi ve otofajide önemi bilinen bir transkripsiyon faktörü olan C/EBPβ (CCAAT/enhancer binding protein beta)nın, kolon hücrelerinin farklılaşma mekanizmasındaki etkinliği araştırılmıştır.
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