Objective: This study was designed to evaluate the prevalence of Human Papilloma Virus (HPV) in semen and document the cycle outcomes in couples with previous intra-cytoplasmic sperm injection (ICSI)failures.Material and methods: One hundred and seventeen couples with at least two ICSI attempts were includedin the study. HPV infection in semen and DNA fragmentation in samples were analyzed by commerciallyavailable kits. The percentage of spermatozoa with fragmented DNA (DNA fragmentation index: DFI) wasdetermined during fluorescence microscopic examination as previously described. The cycle outcomes ofcouples with or without HPV infected male partners were recorded.Results: According to our results, the prevalence of HPV was 7.7% in asymptomatic males with at leasttwo previous ICSI failures. The increased DFI (>30%) was observed in 82.9% of the cases. In HPV-positivecases significantly lower number of good quality embryos were obtained. The implantation and pregnancyrates were similar in infected and non-infected males (p>0.05). The early miscarriage rate was slightlyhigher in HPV- positive group (33% vs. 10%, p>0.05).Conclusion: In cases with previous ICSI failures, the prevalence of HPV infection in semen is not higherthan previously reported infertile populations. The reproductive outcome might be impaired in HPV-positive semen due to lower number of good quality embryos, which needs to be clarified by further largepopulation-based studies.
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Objective: This study aims to document the sperm DNA damage results in infertile male patients with normal-standard semen parameters retrospectively. Material and Methods: The study included samples from 500 male patients with a referred diagnosis of idiopathic infertility and a medical history of at least one assisted reproductive technique (ART) failure. All the patients included in this study were otherwise healthy individuals and had normal-standard semen parameters. We performed retrospective analysis, from the results obtained from samples. Tunel methodology was used, and fluorescence microscopy analysis was performed. From a morphological point of view, apparently normal mature sperms were analyzed. Results: Increased sperm DNA damage rates were detected in our tested population. Advanced age did not correlate with a significant increase in sperm DNA damage ratios in our cohort. Conclusion: The importance of sperm DNA damage should be considered as an etiological factor when evaluating infertile couples. Because the ratio of DNA damage might correlate with various indicators of fertility such as the fertilization rate, embryo cleavage rate, implantation rate, pregnancy rate and live birth rate, it is important to know the ratio of spermatozoa with damaged DNA in the ejaculate in order to predict the fertilization rate and determine the risks that may affect the embryo.
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Şule Mine BAKANAY, Pervin TOPÇUOĞLU, Uğur Aynur BİLGİN,
KANAY YARARBAŞ , Berker Sibel KARAÜZÜM, Muhit ÖZCAN, Mutlu ARAT, Levent ÜNDAR, Osman İLHAN
Aplastik anemi (AA), miyelodisplastik sendrom (MDS) ve akut miyeloblastik lösemi (AML) gibi klonal hastalıklara dönüşüm gösterebilmektedir. Monozomi 7, tedaviyle ilişkili MDS ve sekonder AML ile birlikte sıkça gözlenen kötü prognostik bir kro¬mozomal anomalidir. Bunun yanısıra AA’da lökomojenik transformasyonla da ilişkilendirilmiştir. Burada ilk sıra tedavi olarak immünsupresif tedavi almış ve sırasıyla blast artışıyla giden refrakter anemi (MDS-RAEB-II) ve AML’ye transforme olmuş iki ağır AA’lı olgu bildirmekteyiz. Hastalarda transformasyon sırasında monozomi 7 klonunun pozitif olduğu saptanmıştır. Her iki hastaya da HLA uyumlu vericilerinden (akraba dışı ve kardeş) allojeneik hematopoietik kök hücre transplantasyonu yapılmıştır.
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Ozan PAZVANTOĞLU, İhsan Tuncer OKAY, Nesrin DİLBAZ,
KANAY YARARBAŞ , Işık BÖKESOY
Ozan PAZVANTOĞLU, İhsan Tuncer OKAY, Nesrin DİLBAZ,
KANAY YARARBAŞ ,
Işık BÖKESOY
Klinefelter sendromu, görülme sıklığı fazla olan genetik geçişli bir hastalı ktır. Tipik Şziksel özelliklerinin dışında psikiyatrik belirtilere de sıkça rastlanmaktadır. Bu olgu sunumunda, paranoid içerikli hezeyan ve işitsel algı kusuru gibi psikotik semptomlar nedeniyle başvuran 30 yaşı nda bir olgunun Klinefelter sendromu tanısı alma süreci sunulmuştur. Amacımız hem bu sendromdaki psikiyatrik tabloları gözden geçirmek, hem de psikiyatrik değerlendirmede organik etyolojilerin önemini vurgulamaktır.
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