Önder ÇELİK ,
Şafak HATIRNAZ ,
Aynur ERŞAHİN ,
Alper BAŞBUĞ ,
Gonca Yetkin YILDIRIM , Vahit ÖZENER, Neslihan GÜRPINAR, Sudenaz ÇELİK,
Nilüfer ÇELİK , Tansu KÜÇÜK,
Cihat ÜNLÜ
Objective: To investigate whether prior testis magnetic resonance spectroscopy predicts the success or failure of micro-dissection testicularsperm extraction (micro-TESE) in patients with non-obstructive azoospermia (NOA).Material and Methods: Nine men with NOA who were scheduled for micro-TESE for the first time, 9 NOA men with a history of previousmicro-TESE and 5 fertile men were enrolled. All NOA patients and fertile controls underwent testis spectroscopy. A multi-voxel spectroscopysequence was used. Testicular signals of choline (Cho), creatine (Cr), myo-inositol (MI), lactate, and lipids were analyzed quantitatively andcompared with the results of the micro-TESEs.Results: The most prominent peaks were Cho and Cr in the fertile controls and NOA subjects with positive sperm retrieval in the micro-TESE. Ahigh Cho peak was detected in 87% of the NOA men with positive sperm retrieval. NOA men without sperm at the previous micro-TESE showeda marked decrease in Cho and Cr signals. For positive sperm retrieval in micro-TESE, the cut-off value of Cho was 1.46 ppm, the cut-off value ofCr was 1.43 ppm, and the cut-off value of MI was 0.79 ppm.Conclusion: Testis spectroscopy can be used as a non-invasive screening method to predict the success or failure of micro-TESE. (J Turk GerGynecol Assoc 2020; 21: 70-8)
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Objectives: The aim of this study was to investigate whether first trimester combined screening for major fetaltrisomies is influenced by assisted reproduction techniques (ART) from blastocyst transfer, with or withoutcryopreservation.Methods: This study is a retrospective analysis involving 115 singleton pregnancies with euploid fetusesrecruited between January 2017 and December 2017. Sixty-five women conceived with fresh blastocysts fromin vitro fertilization (IVF) cycles (fresh-blasto), 50 with frozen-thawed blastocysts. All cases underwentultrasound assessment at 11+0 - 13+6 weeks with measurements of crown rump length, nuchal translucency(NT) , free beta-human chorionic gonadotrophin (free β-hCG) and pregnancy-associated plasma protein A(PAPP-A) concentrations.Results: Baseline characteristics and pregnancy outcomes did not differ substantially among the study groups.The NT was not significantly different in the frozen-thawed -blasto compared to the fresh-blasto group (p =0.741). The free β-hCG levels was not significantly different in frozen-thawed-blasto group compared to freshblasto group (p = 0.495). The two groups showed no significant difference in the PAPP-A levels (p = 0,139).The median delta crown rump length was also not significantly different among the two groups (p = 0.758).Conclusions: In ART pregnancies from blastocyst transfer, with or without cryopreservation, the NTmeasurement, free β-hCG concentration and PAPP-A levels did not show any significant difference. Thesefeatures are apparently unrelated to the outcome of pregnancy and may be due to alterations or delays inembryogenesis or placentation with potential relevance for the screening test performance.
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